RT Journal Article
T1 The extracellular matrix protects Bacillus subtilis colonies from Pseudomonas invasion and modulates plant co-colonization.
A1 Molina-Santiago, Carlos
A1 Pearson, John R
A1 Navarro, Yurena
A1 Berlanga-Clavero, María Victoria
A1 Caraballo-Rodriguez, Andrés Mauricio
A1 Petras, Daniel
A1 García-Martín, María Luisa
A1 Lamon, Gaelle
A1 Haberstein, Birgit
A1 Cazorla, Francisco M
A1 de Vicente, Antonio
A1 Loquet, Antoine
A1 Dorrestein, Pieter C
A1 Romero, Diego
AB Bacteria of the genera Pseudomonas and Bacillus can promote plant growth and protect plants from pathogens. However, the interactions between these plant-beneficial bacteria are understudied. Here, we explore the interaction between Bacillus subtilis 3610 and Pseudomonas chlororaphis PCL1606. We show that the extracellular matrix protects B. subtilis colonies from infiltration by P. chlororaphis. The absence of extracellular matrix results in increased fluidity and loss of structure of the B. subtilis colony. The P. chlororaphis type VI secretion system (T6SS) is activated upon contact with B. subtilis cells, and stimulates B. subtilis sporulation. Furthermore, we find that B. subtilis sporulation observed prior to direct contact with P. chlororaphis is mediated by histidine kinases KinA and KinB. Finally, we demonstrate the importance of the extracellular matrix and the T6SS in modulating the coexistence of the two species on melon plant leaves and seeds.
YR 2019
FD 2019-04-23
LK http://hdl.handle.net/10668/13871
UL http://hdl.handle.net/10668/13871
LA en
DS RISalud
RD Apr 18, 2025