RT Journal Article
T1 Allergens Induce the Release of Lactoferrin by Neutrophils from Asthmatic Patients.
A1 Fernández-Delgado, Lourdes
A1 Vega-Rioja, Antonio
A1 Ventura, Inmaculada
A1 Chamorro, Cristina
A1 Aroca, Rocío
A1 Prados, Manuel
A1 Bobadilla, Pedro
A1 Rodríguez, David
A1 Palacios, Ricardo
A1 Monteseirín, Javier
K1 Alérgenos
K1 Anticuerpos monoclonales
K1 Asma
K1 Galectina 3
K1 Inmunosupresores
AB BACKGROUNDDespite the evidence that Lactoferrin (Lf) is involved in allergic asthma processes, it is unknown whether neutrophils can be one of the main cellular sources of this key inflammatory mediator directly in response of an IgE mediated stimulus. The present study was undertaken to analyze this question.METHODSNeutrophils from healthy subjects (n = 34) and neutrophils from allergic asthmatic patients (n = 102) were challenged in vitro with specific allergens to which the patients were sensitized, PAF, or agonist mAbs against IgE-receptors, and the levels of Lf were measured in the culture supernatant. The levels of serum IgE together with the severity of symptoms were also analyzed.RESULTSLf was released into the culture supernatant of neutrophils from allergic asthmatic patients in response to allergens and PAF. This response was highly allergen-specific, and did not happen in neutrophils from healthy donors. Allergen effect was mimicked by Abs against FcεRI and galectin-3 but not by FcεRII. The levels of released Lf correlated well with the levels of serum specific IgE and severity of asthma symptoms. These observations represent a novel view of neutrophils as an important source of Lf in allergic asthma. Importantly, the levels of released Lf by neutrophils could therefore be used to evaluate disease severity in allergic asthmatic patients.
PB Public Library of Science
YR 2015
FD 2015-10-21
LK http://hdl.handle.net/10668/2104
UL http://hdl.handle.net/10668/2104
LA en
NO Fernández-Delgado L, Vega-Rioja A, Ventura I, Chamorro C, Aroca R, Prados M, et al. Allergens Induce the Release of Lactoferrin by Neutrophils from Asthmatic Patients. PLoS ONE. 2015; 10(10):e0141278
NO Journal Article;
DS RISalud
RD Apr 17, 2025