RT Journal Article
T1 Impact of Vitamin D Deficit on the Rat Gut Microbiome.
A1 Robles-Vera, Iñaki
A1 Callejo, María
A1 Ramos, Ricardo
A1 Duarte, Juan
A1 Perez-Vizcaino, Francisco
K1 16S rRNA sequencing
K1 microbiota
K1 vitamin D deficit
AB Inadequate immunologic, metabolic and cardiovascular homeostasis has been related to either an alteration of the gut microbiota or to vitamin D deficiency. We analyzed whether vitamin D deficiency alters rat gut microbiota. Male Wistar rats were fed a standard or a vitamin D-free diet for seven weeks. The microbiome composition was determined in fecal samples by 16S rRNA gene sequencing. The vitamin D-free diet produced mild changes on α- diversity but no effect on β-diversity in the global microbiome. Markers of gut dysbiosis like Firmicutes-to-Bacteroidetes ratio or the short chain fatty acid producing bacterial genera were not significantly affected by vitamin D deficiency. Notably, there was an increase in the relative abundance of the Enterobacteriaceae, with significant rises in its associated genera Escherichia, Candidatus blochmannia and Enterobacter in vitamin D deficient rats. Prevotella and Actinomyces were also increased and Odoribacteraceae and its genus Butyricimonas were decreased in rats with vitamin D-free diet. In conclusion, vitamin D deficit does not induce gut dysbiosis but produces some specific changes in bacterial taxa, which may play a pathophysiological role in the immunologic dysregulation associated with this hypovitaminosis.
YR 2019
FD 2019-10-24
LK http://hdl.handle.net/10668/15389
UL http://hdl.handle.net/10668/15389
LA en
DS RISalud
RD Apr 11, 2025