%0 Journal Article
%A Losada Méndez, Jorge
%A Palomares, Francisca
%A Gómez, Francisca
%A Ramírez-López, Pedro
%A Ramos-Soriano, Javier
%A Torres, Maria Jose
%A Mayorga, Cristobalina
%A Rojo, Javier
%T Immunomodulatory Response of Toll-like Receptor Ligand-Peptide Conjugates in Food Allergy.
%D 2021
%U https://hdl.handle.net/10668/24540
%X Covalent conjugation of allergens to toll-like receptor (TLR) agonists appears to be a powerful strategy for the development of safety compounds for allergen-specific immunomodulatory response toward tolerance in allergy. In this work, we have synthesized two family of ligands, an 8-oxoadenine derivative as a ligand for TLR7 and a pyrimido[5,4-b]indole as a ligand for TLR4, both conjugated with a T-cell peptide of Pru p 3 allergen, the lipid transfer protein (LTP) responsible for LTP-dependent food allergy. These conjugates interact with dendritic cells, inducing their specific maturation, T-cell proliferation, and cytokine production in peach allergic patients. Moreover, they increased the Treg-cell frequencies in these patients and could induce the IL-10 production. These outcomes were remarkable in the case of the TLR7 ligand conjugated with Pru p 3, opening the door for the potential application of these allergen-adjuvant systems in food allergy immunotherapy.
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