RT Journal Article
T1 Toxicity Induced by Cytokines, Glucose, and Lipids Increase Apoptosis and Hamper Insulin Secretion in the 1.1E7 Beta Cell-Line
A1 Diaz-Ganete, Antonia
A1 Quiroga-de-Castro, Aranzazu
A1 Mateos, Rosa M.
A1 Medina, Francisco
A1 Segundo, Carmen
A1 Lechuga-Sancho, Alfonso M.
K1 Apoptosis
K1 Beta cell
K1 Cytokines
K1 Cytotoxicity
K1 Diabetes
K1 Glucotoxicity
K1 Lipotoxicity
K1 Mechanism
K1 Células secretoras de insulina
K1 Citocinas
K1 Citotoxicidad inmunológica
K1 Glucosa
K1 Lípidos
K1 Toxicidad
AB Basic research on types 1 and 2 diabetes mellitus require early stage studies using beta cells or cell lines, ideally of human origin and with preserved insulin secretion in response to glucose. The 1.1E7 cells are a hybrid cell line resulting from the electrofusion of dispersed human islets and PANC-1 cells, capable of secreting insulin in response to glucose, but their survival and function under toxic conditions remains untested. This characterization is the purpose of the present study. We treated these cells with a cytokine mix, high glucose, palmitate, and the latter two combined. Under these conditions, we measured cell viability and apoptosis (MTT, Caspase Glo and TUNEL assays, as well as caspase-8 and -9 levels by Western blotting), endoplasmic reticulum stress markers (EIF2AK3, HSPA4, EIF2a, and HSPA5) by real-time PCR, and insulin secretion with a glucose challenge. All of these stimuli (i) induce apoptosis and ER stress markers expression, (ii) reduce mRNA amounts of 2-5 components of genes involved in the insulin secretory pathway, and (iii) abrogate the insulin release capability of 1.1E7 cells in response to glucose. The most pronounced effects were observed with cytokines and with palmitate and high glucose combined. This characterization may well serve as the starting point for those choosing this cell line for future basic research on certain aspects of diabetes.
PB MDPI
SN 1661-6596
YR 2021
FD 2021-03-04
LK http://hdl.handle.net/10668/4497
UL http://hdl.handle.net/10668/4497
LA en
NO Diaz-Ganete A, Quiroga-de-Castro A, Mateos RM, Medina F, Segundo C, Lechuga-Sancho AM. Toxicity Induced by Cytokines, Glucose, and Lipids Increase Apoptosis and Hamper Insulin Secretion in the 1.1E7 Beta Cell-Line. Int J Mol Sci. 2021 Mar 4;22(5):2559
DS RISalud
RD Apr 14, 2025