RT Journal Article
T1 Quantitative analysis of protein-RNA interactions in fission yeast.
A1 Elías-Villalobos, Alberto
A1 Duncan, Caia
A1 Mata, Juan
A1 Helmlinger, Dominique
K1 Model Organisms
K1 Molecular Biology
K1 Protein Biochemistry
AB Characterizing the interactions between RNAs and proteins in vivo is key to better understand how organisms regulate gene expression. Here, we describe a robust and quantitative protocol to measure specific RNA-protein interactions in a native context using RNA immunoprecipitation (RIP). We provide a comprehensive experimental framework to detect cotranslational interactions and detail the quantitative analysis of purified RNAs by PCR and high-throughput sequencing. Although we developed the protocol in fission yeast, it can be readily implemented in other yeast species. For complete details on the use and execution of this protocol, please refer to Toullec et al. (2021).
YR 2022
FD 2022-05-09
LK http://hdl.handle.net/10668/22543
UL http://hdl.handle.net/10668/22543
LA en
DS RISalud
RD Apr 10, 2025