%0 Journal Article
%A Lopez-Baltanas, Rodrigo
%A Encarnacion Rodriguez-Ortiz, Maria
%A Canalejo, Antonio
%A Diaz-Tocados, Juan M
%A Herencia, Carmen
%A Leiva-Cepas, Fernando
%A Torres-Peña, Jose D
%A Ortiz-Morales, Ana
%A Muñoz-Castañeda, Juan Rafael
%A Rodriguez, Mariano
%A Almaden, Yolanda
%T Magnesium supplementation reduces inflammation in rats with induced chronic kidney disease.
%D 2021
%U http://hdl.handle.net/10668/17599
%X Inflammation is a common feature in chronic kidney disease (CKD) that appears specifically associated with cardiovascular derangements in CKD patients. Observational studies have revealed a link between low Mg levels and inflammation. In this study, we hypothesize that Mg might have a modulatory effect on the inflammation induced under the uraemic milieu. In vivo studies were performed in a 5/6 nephrectomized rat model of CKD. Furthermore, a possible direct effect of Mg was addressed through in vitro studies with vascular smooth muscle cells (VSMCs). Uraemic rats fed a normal (0.1%) Mg diet showed a systemic inflammatory response evidenced by the elevation in plasma of the pro-inflammatory cytokines TNF-α, IL-1β and IL-6, and GPx activity, a marker of oxidative stress. Importantly, an increased expression of these cytokines in the aortic tissue was also observed. In contrast, a dietary Mg supplementation (0.6%) greatly prevented the oxidative stress and the pro-inflammatory response. In vitro, in VSMCs cultured in a pro-inflammatory high phosphate medium, incubation with Mg 1.6 mM inhibited the increase in the production of ROS, the rise in the expression of TNF-α, IL-1β, IL-6 and IL-8 and the activation of NF-κB signalling that was observed in cells incubated with a normal (0.8 mM) Mg. Mg supplementation reduced inflammation associated with CKD, exerting a direct effect on vascular cells. These findings support a possible beneficial effect of Mg supplementation along the clinical management of CKD patients.
%K Chronic kidney disease
%K Inflammation
%K Magnesium
%K Phosphate
%K Uraemia
%K Vascular smooth muscle cells
%~