RT Journal Article
T1 Circadian Rhythms in Hormone-sensitive Lipase in Human Adipose Tissue: Relationship to Meal Timing and Fasting Duration.
A1 Arredondo-Amador, Maria
A1 Zambrano, Carolina
A1 Kulyte, Agne
A1 Lujan, Juan
A1 Hu, Kun
A1 Sanchez de Medina, Fermin
A1 Scheer, Frank A J L
A1 Arner, Peter
A1 Ryden, Mikael
A1 Martinez-Augustin, Olga
A1 Garaulet, Marta
K1 insulin
K1 lipolysis
K1 obesity
K1 time-restricting eating
AB Fat mobilization in adipose tissue (AT) has a specific timing. However, circadian rhythms in the activity of the major enzyme responsible for fat mobilization, hormone-sensitive lipase (HSL), have not been demonstrated in humans. To analyze in a cross-sectional study whether there is an endogenous circadian rhythm in HSL activity in human AT ex vivo and whether rhythm characteristics are related to food timing or fasting duration. Abdominal AT biopsies were obtained from 18 severely obese participants (age: 46 ± 11 years; body mass index 42 ± 6 kg/m2) who underwent laparoscopic gastric bypass. Twenty-four-hour rhythms of HSL activity and LIPE (HSL transcript in humans) expression in subcutaneous AT were analyzed together with habitual food timing and night fasting duration. HSL activity exhibited a circadian rhythm (P = .023) and reached the maximum value at circadian time 16 (CT) that corresponded to around midnight (relative local clock time. Similarly, LIPE displayed a circadian rhythm with acrophase also at night (P = .0002). Participants with longer night fasting duration >11.20 hours displayed almost double the amplitude (1.91 times) in HSL activity rhythm than those with short duration (P = .013); while habitual early diners (before 21:52 hours) had 1.60 times higher amplitude than late diners (P = .035). Our results demonstrate circadian rhythms in HSL activity and may lead to a better understanding of the intricate relationships between food timing, fasting duration and body fat regulation.
PB Oxford University Press
YR 2020
FD 2020-07-27
LK http://hdl.handle.net/10668/16022
UL http://hdl.handle.net/10668/16022
LA en
NO Arredondo-Amador M, Zambrano C, Kulyté A, Luján J, Hu K, Sánchez de Medina F, et al. Circadian Rhythms in Hormone-sensitive Lipase in Human Adipose Tissue: Relationship to Meal Timing and Fasting Duration. J Clin Endocrinol Metab. 2020 Dec 1;105(12):e4407–16.
NO This work has been supported in part by the Spanish Government of Investigation, Development and Innovation (SAF2017-84135-R to M.G., SAF2017-88457-R to O.M., AGL2017-85270-R OM) including FEDER cofunding; the Autonomous Community of the Region of Murcia through the Seneca Foundation (20795/PI/18) to M.G., Junta de Andalucía (CTS235, CTS164) to O.M. and F.S.M., and NIDDK R01DK105072 to M.G.. F.A.J.L.S. was supported in part by NIDDK R01DK105072 and NHLBI R01HL140574. M.A.A. was supported by the Ministry of Education, Culture and Sport (Spain). CIBERehd is funded by Instituto de Salud Carlos III. K.H. was supported in part by RF1AG059867 and National Institute of Aging (NIA) RF1AG064312.
DS RISalud
RD Apr 19, 2025