RT Journal Article
T1 Generation of a novel human dermal substitute functionalized with antibiotic-loaded nanostructured lipid carriers (NLCs) with antimicrobial properties for tissue engineering.
A1 Chato-Astrain, Jesús
A1 Chato-Astrain, Isabel
A1 Sánchez-Porras, David
A1 García-García, Óscar-Darío
A1 Bermejo-Casares, Fabiola
A1 Vairo, Claudia
A1 Villar-Vidal, María
A1 Gainza, Garazi
A1 Villullas, Silvia
A1 Oruezabal, Roke-Iñaki
A1 Ponce-Polo, Ángela
A1 Garzón, Ingrid
A1 Carriel, Víctor
A1 Campos, Fernando
A1 Alaminos, Miguel
K1 Amikacin
K1 Colistimethate
K1 Dermal substitute
K1 Functionalization
K1 Human skin
K1 Nanostructured lipid carriers
K1 Severe burns
K1 Tissue engineering
AB Treatment of patients affected by severe burns is challenging, especially due to the high risk of Pseudomonas infection. In the present work, we have generated a novel model of bioartificial human dermis substitute by tissue engineering to treat infected wounds using fibrin-agarose biomaterials functionalized with nanostructured lipid carriers (NLCs) loaded with two anti-Pseudomonas antibiotics: sodium colistimethate (SCM) and amikacin (AMK). Results show that the novel tissue-like substitutes have strong antibacterial effect on Pseudomonas cultures, directly proportional to the NLC concentration. Free DNA quantification, WST-1 and Caspase 7 immunohistochemical assays in the functionalized dermis substitute demonstrated that neither cell viability nor cell proliferation were affected by functionalization in most study groups. Furthermore, immunohistochemistry for PCNA and KI67 and histochemistry for collagen and proteoglycans revealed that cells proliferated and were metabolically active in the functionalized tissue with no differences with controls. When functionalized tissues were biomechanically characterized, we found that NLCs were able to improve some of the major biomechanical properties of these artificial tissues, although this strongly depended on the type and concentration of NLCs. These results suggest that functionalization of fibrin-agarose human dermal substitutes with antibiotic-loaded NLCs is able to improve the antibacterial and biomechanical properties of these substitutes with no detectable side effects. This opens the door to future clinical use of functionalized tissues.
YR 2020
FD 2020-11-23
LK http://hdl.handle.net/10668/16656
UL http://hdl.handle.net/10668/16656
LA en
DS RISalud
RD Apr 10, 2025