RT Journal Article
T1 Next-generation sequencing and genotype association studies reveal the association of HLA-DRB3*02:02 with delayed hypersensitivity to penicillins
A1 Romano, Antonino
A1 Oussalah, Abderrahim
A1 Chery, Celine
A1 Gueant-Rodriguez, Rosa-Maria
A1 Gaeta, Francesco
A1 Cornejo-Garcia, Jose-Antonio
A1 Rouyer, Pierre
A1 Josse, Thomas
A1 Mayorga, Cristobalina
A1 Torres, Maria-Jose
A1 Gueant, Jean-Louis
K1 amoxicillin
K1 beta-lactams
K1 drug allergy
K1 genome-wide association
K1 HLA-DRB3
K1 nonimmediate reactions
K1 penicillins
K1 Allergic reactions
K1 Variants
K1 Linkage
K1 Domain
AB Background Nonimmediate (delayed)-allergic reactions to penicillins are common and some of them can be life-threatening. The genetic factors influencing these reactions are unknown/poorly known/poorly understood. We assessed the genetic predictors of a delayed penicillin allergy that cover the HLA loci. Methods Using next-generation sequencing (NGS), we genotyped the MHC region in 24 patients with delayed hypersensitivity compared with 20 patients with documented immediate hypersensitivity to penicillins recruited in Italy. Subsequently, we analyzed in silico Illumina Immunochip genotyping data that covered the HLA loci in 98 Spanish patients with delayed hypersensitivity and 315 with immediate hypersensitivity compared to 1,308 controls. Results The two alleles DRB3*02:02:01:02 and DRB3*02:02:01:01 were reported in twenty cases with delayed reactions (83%) and ten cases with immediate reactions (50%), but not in the Allele Frequency Net Database. Bearing at least one of the two alleles increased the risk of delayed reactions compared to immediate reactions, with an OR of 8.88 (95% CI, 3.37-23.32; p
PB Wiley
SN 0105-4538
YR 2021
FD 2021-11-14
LK https://hdl.handle.net/10668/24391
UL https://hdl.handle.net/10668/24391
LA en
DS RISalud
RD Apr 17, 2025