RT Journal Article
T1 Bisphenol F and bisphenol S promote lipid accumulation and adipogenesis in human adipose-derived stem cells
A1 Reina-Perez, I
A1 Olivas-Martinez, A
A1 Mustieles, V
A1 Ruiz-Ojeda, F J
A1 Molina-Molina, J M
A1 Olea, N
A1 Fernandez, M F
K1 Humans
K1 Adipogenesis
K1 PPAR gamma
K1 Fulvestrant
K1 Lipids
AB Bisphenol F (BPF) and bisphenol S (BPS) are increasingly used as substitutes for bisphenol A (BPA), an endocrine disrupting chemical (EDC) with obesogenic activity. We investigated the in vitro effects of BPS and BPF on the adipogenesis of human adipose-derived stem cells (hASCs) exposed to different doses (0.01, 0.1, 1, 10 and 25 μM), stopping the adipogenic process at 7 or 14 days. Intracellular lipid accumulation was quantified by the Oil Red O assay, gene expression of peroxisome proliferator-activated receptor gamma (PPARγ), CCAT/enhancer-binding protein (C/EBPα), lipoprotein-lipase (LPL) and fatty acid binding protein 4 (FABP4), by quantitative real-time polymerase chain reaction (qRT-PCR) and protein levels by Western Blot. hASCs with BPF or BPS produced a linear dose-response increase in intracellular lipid accumulation and in gene expression of the adipogenic markers, confirmed by protein levels. Co-treatment ICI 182,780 significantly inhibited BPF- but not BPS-induced lipid accumulation. Given the affinity of bisphenols for diverse nuclear receptors, their obesogenic effects may result from a combination of pathways rather than a single mechanism. Further research is warranted on the manner in which chemicals interfere with adipogenic differentiation. To our best knowledge, this report shows for the first time the obesogenic potential of BPF in hASCs.
YR 2021
FD 2021-04-11
LK http://hdl.handle.net/10668/22229
UL http://hdl.handle.net/10668/22229
LA en
NO Reina-Pérez I, Olivas-Martínez A, Mustieles V, Ruiz-Ojeda FJ, Molina-Molina JM, Olea N, et al. Corrigendum to "Bisphenol F and bisphenol S promote lipid accumulation and adipogenesis in human adipose-derived stem cells" [Food Chem Toxicol. 152 (2021 Jun) 112216]. Food Chem Toxicol. 2022 Aug;166:113237.
DS RISalud
RD Apr 12, 2025