RT Journal Article
T1 Selective Engineering of Linkage-Specific α2,6-N-Linked Sialoproteins Using Sydnone-Modified Sialic Acid Bioorthogonal Reporters.
A1 Chinoy, Zoeisha S
A1 Bodineau, Clément
A1 Favre, Camille
A1 Moremen, Kelley W
A1 Durán, Raúl V
A1 Friscourt, Frédéric
K1 click chemistry
K1 enzymes
K1 fluorescent probes
K1 glycobiology
K1 sialic acids
AB The metabolic oligosaccharide engineering (MOE) strategy using unnatural sialic acids has recently enabled the visualization of the sialome in living systems. However, MOE only reports on global sialylation and dissected information regarding subsets of sialosides is missing. Described here is the synthesis and utilization of sialic acids modified with a sydnone reporter for the metabolic labeling of sialoconjugates. The positioning of the reporter on the sugar significantly altered its metabolic fate. Further in vitro enzymatic assays revealed that the 9-modified neuraminic acid is preferentially accepted by the sialyltransferase ST6Gal-I over ST3Gal-IV, leading to the favored incorporation of the reporter into linkage-specific α2,6-N-linked sialoproteins. This sydnone sugar presents the possibility of investigating the roles of specific sialosides.
YR 2019
FD 2019-02-19
LK http://hdl.handle.net/10668/13493
UL http://hdl.handle.net/10668/13493
LA en
DS RISalud
RD Apr 11, 2025