RT Journal Article T1 Molecularly determined total tumour load in lymph nodes of stage I-II colon cancer patients correlates with high-risk factors. A multicentre prospective study. A1 Aldecoa, Iban A1 Atares, Begoña A1 Tarragona, Jordi A1 Bernet, Laia A1 Sardon, Jose Domingo A1 Pereda, Teresa A1 Villar, Carlos A1 Mendez, M Carmen A1 Gonzalez-Obeso, Elvira A1 Elorriaga, Kepa A1 Alonso, Guadalupe Lopez A1 Zamora, Javier A1 Planell, Nuria A1 Palacios, Jose A1 Castells, Antoni A1 Matias-Guiu, Xavier A1 Cuatrecasas, Miriam K1 Colorectal neoplasms K1 Cytokeratin 19 K1 Lymph nodes K1 Molecular pathology K1 Neoplasm staging AB Stage I-II (pN0) colorectal cancer patients are surgically treated although up to 25 % will eventually die from disease recurrence. Lymph node (LN) status is an independent prognostic factor in colorectal cancer (CRC), and molecular tumour detection in LN of early-stage CRC patients is associated with an increased risk of disease recurrence and poor survival. This prospective multicentre study aimed to determine the relationship between LN molecular tumour burden and conventional high-risk factors in stage I-II colon cancer patients. A total of 1940 LN from 149 pathologically assessed pN0 colon cancer patients were analysed for the amount of tumour cytokeratin 19 (CK19) messenger RNA (mRNA) with the quantitative reverse transcription loop-mediated isothermal amplification molecular assay One-Step Nucleic Acid Amplification. Patient's total tumour load (TTL) resulted from the sum of all CK19 mRNA tumour copies/μL of each positive LN from the colectomy specimen. A median of 15 LN were procured per case (IQR 12;20). Molecular positivity correlated with high-grade (p  YR 2016 FD 2016-07-22 LK http://hdl.handle.net/10668/10300 UL http://hdl.handle.net/10668/10300 LA en DS RISalud RD Apr 18, 2025