RT Journal Article
T1 Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot.
A1 Humbert, Magali
A1 Morán, María
A1 de la Cruz-Ojeda, Patricia
A1 Muntané, Jordi
A1 Wiedmer, Tabea
A1 Apostolova, Nadezda
A1 McKenna, Sharon L
A1 Velasco, Guillermo
A1 Balduini, Walter
A1 Eckhart, Leopold
A1 Janji, Bassam
A1 Sampaio-Marques, Belém
A1 Ludovico, Paula
A1 Žerovnik, Eva
A1 Langer, Rupert
A1 Perren, Aurel
A1 Engedal, Nikolai
A1 Tschan, Mario P
K1 autophagy
K1 biomarkers
K1 disease
K1 pathology
AB Autophagy is a highly conserved degradation mechanism that is essential for maintaining cellular homeostasis. In human disease, autophagy pathways are frequently deregulated and there is immense interest in targeting autophagy for therapeutic approaches. Accordingly, there is a need to determine autophagic activity in human tissues, an endeavor that is hampered by the fact that autophagy is characterized by the flux of substrates whereas histology informs only about amounts and localization of substrates and regulators at a single timepoint. Despite this challenging task, considerable progress in establishing markers of autophagy has been made in recent years. The importance of establishing clear-cut autophagy markers that can be used for tissue analysis cannot be underestimated. In this review, we attempt to summarize known techniques to quantify autophagy in human tissue and their drawbacks. Furthermore, we provide some recommendations that should be taken into consideration to improve the reliability and the interpretation of autophagy biomarkers in human tissue samples.
SN 2079-7737
YR 2020
FD 2020-03-21
LK https://hdl.handle.net/10668/28403
UL https://hdl.handle.net/10668/28403
LA en
DS RISalud
RD Apr 5, 2025