TY - JOUR AU - Ruiz-Ruiz, Susana AU - Ponce, Carolina A AU - Pesantes, Nicole AU - Bustamante, Rebeca AU - Gatti, Gianna AU - San Martin, Viviana AU - Gutierrez, Mireya AU - Borquez, Pamela AU - Vargas, Sergio L AU - Magne, Fabien AU - Calderon, Enrique J AU - Perez-Brocal, Vicente AU - Moya, Andres PY - 2022 DO - 10.3389/fmicb.2021.787554 SN - 1664-302X UR - http://hdl.handle.net/10668/20615 T2 - Frontiers in microbiology AB - Here we report a new real-time PCR assay using SYBR Green which provides higher sensitivity for the specific detection of low levels of Pneumocystis jirovecii. To do so, two primer sets were designed, targeting the family of genes that code for the... LA - en PB - Frontiers Research Foundation KW - low P. jirovecii loads KW - major surface glycoproteins KW - mitochondrial large subunit rRNA KW - real-time PCR KW - simultaneous detection KW - Pneumocystis carinii KW - Pneumocystis KW - Real-Time Polymerase Chain Reaction KW - SYBR Green I KW - Membrane Glycoproteins KW - Membrane Proteins KW - Pneumonia, Pneumocystis TI - A Real-Time PCR Assay for Detection of Low Pneumocystis jirovecii Levels. TY - research article VL - 12 ER -