RT Journal Article
T1 Spanish multicenter study of the epidemiology and mechanisms of amoxicillin-clavulanate resistance in Escherichia coli.
A1 Ortega, Adriana
A1 Oteo, Jesús
A1 Aranzamendi-Zaldumbide, Maitane
A1 Bartolomé, Rosa M
A1 Bou, Germán
A1 Cercenado, Emilia
A1 Conejo, M Carmen
A1 González-López, Juan José
A1 Marín, Mercedes
A1 Martínez-Martínez, Luis
A1 Merino, María
A1 Navarro, Ferran
A1 Oliver, Antonio
A1 Pascual, Alvaro
A1 Rivera, Alba
A1 Rodríguez-Baño, Jesús
A1 Weber, Irene
A1 Aracil, Belén
A1 Campos, José
K1 Combinación de amoxicilina clavulanato potásico
K1 Farmacorresistencia bacteriana
K1 Infecciones por Escherichia coli
K1 Epidemiología molecular
K1 Estudios prospectivos
K1 España
AB We conducted a prospective multicenter study in Spain to characterize the mechanisms of resistance to amoxicillin-clavulanate (AMC) in Escherichia coli. Up to 44 AMC-resistant E. coli isolates (MIC ≥ 32/16 μg/ml) were collected at each of the seven participant hospitals. Resistance mechanisms were characterized by PCR and sequencing. Molecular epidemiology was studied by pulsed-field gel electrophoresis (PFGE) and by multilocus sequence typing. Overall AMC resistance was 9.3%. The resistance mechanisms detected in the 257 AMC-resistant isolates were OXA-1 production (26.1%), hyperproduction of penicillinase (22.6%), production of plasmidic AmpC (19.5%), hyperproduction of chromosomic AmpC (18.3%), and production of inhibitor-resistant TEM (IRT) (17.5%). The IRTs identified were TEM-40 (33.3%), TEM-30 (28.9%), TEM-33 (11.1%), TEM-32 (4.4%), TEM-34 (4.4%), TEM-35 (2.2%), TEM-54 (2.2%), TEM-76 (2.2%), TEM-79 (2.2%), and the new TEM-185 (8.8%). By PFGE, a high degree of genetic diversity was observed although two well-defined clusters were detected in the OXA-1-producing isolates: the C1 cluster consisting of 19 phylogroup A/sequence type 88 [ST88] isolates and the C2 cluster consisting of 19 phylogroup B2/ST131 isolates (16 of them producing CTX-M-15). Each of the clusters was detected in six different hospitals. In total, 21.8% of the isolates were serotype O25b/phylogroup B2 (O25b/B2). AMC resistance in E. coli is widespread in Spain at the hospital and community levels. A high prevalence of OXA-1 was found. Although resistant isolates were genetically diverse, clonality was linked to OXA-1-producing isolates of the STs 88 and 131. Dissemination of IRTs was frequent, and the epidemic O25b/B2/ST131 clone carried many different mechanisms of AMC resistance.
PB American Society for Microbiology
SN 0066-4804
YR 2012
FD 2012-07
LK http://hdl.handle.net/10668/1901
UL http://hdl.handle.net/10668/1901
LA en
NO Ortega A, Oteo J, Aranzamendi-Zaldumbide M, Bartolomé RM, Bou G, Cercenado E, et al. Spanish multicenter study of the epidemiology and mechanisms of amoxicillin-clavulanate resistance in Escherichia coli. Antimicrob. Agents Chemother.. 2012 ; 56(7):3576-81
NO Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 3, 2025