RT Journal Article
T1 Nalmefene is effective at reducing alcohol seeking, treating alcohol-cocaine interactions and reducing alcohol-induced histone deacetylases gene expression in blood.
A1 Calleja-Conde, Javier
A1 Echeverry-Alzate, Victor
A1 Gine, Elena
A1 Buhler, Kora-Mareen
A1 Nadal, Roser
A1 Maldonado, Rafael
A1 Rodriguez-de-Fonseca, Fernando
A1 Gual, Antoni
A1 Lopez-Moreno, Jose Antonio
K1 Alcohol Drinking
K1 Cocaine
K1 Dose-Response Relationship, Drug
K1 Drug Interactions
K1 Ethanol
AB The opioid antagonist nalmefene (selincro®) was approved for alcohol-related disorders by the European Medicines Agency in 2013. However, there have been no studies regarding the effectiveness of nalmefene when alcohol is used in combination with cocaine. Using operant alcohol self-administration in Wistar rats and qRT-PCR, we evaluated (i) the dose-response curve for s.c. and p.o. nalmefene; (ii) the effects of nalmefene with increasing concentrations of alcohol; (iii) the efficacy of nalmefene on cocaine-potentiated alcohol responding; and (iv) the gene expression profiles of histone deacetylases (Hdac1-11) in peripheral blood in vivo and in the prefrontal cortex, heart, liver and kidney post mortem. S.c. (0.01, 0.05, 0.1 mg·kg(-1) ) and p.o. (10, 20, 40 mg·kg(-1) ) nalmefene dose-dependently reduced alcohol-reinforced responding by up to 50.3%. This effect of nalmefene was not dependent on alcohol concentration (10, 15, 20%). Cocaine potentiated alcohol responding by approximately 40% and nalmefene (0.05 mg·kg(-1) ) reversed this effect of cocaine. Alcohol increased Hdac gene expression in blood and nalmefene prevented the increases in Hdacs 3, 8, 5, 7, 9, 6 and 10. In the other tissues, alcohol and nalmefene either did not alter the gene expression of Hdacs, as in the prefrontal cortex, or a tissue-Hdac-specific effect was observed. Nalmefene might be effective as a treatment for alcohol-dependent patients who also use cocaine. Also, the expression of Hdacs in peripheral blood might be useful as a biomarker of alcohol use and drug response.
PB John Wiley & Sons
YR 2016
FD 2016-07-25
LK http://hdl.handle.net/10668/10134
UL http://hdl.handle.net/10668/10134
LA en
NO Calleja-Conde J, Echeverry-Alzate V, Giné E, Bühler KM, Nadal R, Maldonado R, et al. Nalmefene is effective at reducing alcohol seeking, treating alcohol-cocaine interactions and reducing alcohol-induced histone deacetylases gene expression in blood. Br J Pharmacol. 2016 Aug;173(16):2490-505
NO This work was supported by The European Foundation for Al-cohol Research (to J.A.L.M., F.R.d.F., R.M. and R.N.), theFondo de Investigación Sanitaria (Red de TrastornosAdictivos, FEDER, RD12/0028/0015 to J.A.L.M., RD12/0028/001 to F.R.d.F., RD12/0028/023 to R.M., RD12/0028/0014 toR.N.) and Ministerio de Ciencia e Innovación (SAF2011-26818 to J.A.L.M.)
DS RISalud
RD May 10, 2025