%0 Journal Article
%A Bruzelius, Andreas
%A Hidalgo, Isabel
%A Boza-Serrano, Antonio
%A Hjelmer, Anna-Giorgia
%A Tison, Amelie
%A Deierborg, Tomas
%A Bengzon, Johan
%A Ramos-Moreno, Tania
%T The human bone marrow harbors a CD45- CD11B+ cell progenitor permitting rapid microglia-like cell derivative approaches.
%D 2021
%U http://hdl.handle.net/10668/16762
%X Microglia, the immune sentinel of the central nervous system (CNS), are generated from yolk sac erythromyeloid progenitors that populate the developing CNS. Interestingly, a specific type of bone marrow-derived monocyte is able to express a yolk sac microglial signature and populate CNS in disease. Here we have examined human bone marrow (hBM) in an attempt to identify novel cell sources for generating microglia-like cells to use in cell-based therapies and in vitro modeling. We demonstrate that hBM stroma harbors a progenitor cell that we name stromal microglial progenitor (STR-MP). STR-MP single-cell gene analysis revealed the expression of the consensus genetic microglial signature and microglial-specific genes present in development and CNS pathologies. STR-MPs can be expanded and generate microglia-like cells in vitro, which we name stromal microglia (STR-M). STR-M cells show phagocytic ability, classically activate, and survive and phagocyte in human brain tissue. Thus, our results reveal that hBM harbors a source of microglia-like precursors that can be used in patient-centered fast derivative approaches.
%K bone marrow
%K common myeloid progenitor
%K human bone marrow
%K microglia
%K microglia-like cell in vitro model
%K microglial precursor
%K pluripotent stem cell
%K primitive myeloid progenitor
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