RT Journal Article
T1 Identification of serum microRNAs as potential biomarkers in Pompe disease.
A1 Carrasco-Rozas, Ana
A1 Fernández-Simón, Esther
A1 Lleixà, Maria Cinta
A1 Belmonte, Izaskun
A1 Pedrosa-Hernandez, Irene
A1 Montiel-Morillo, Elena
A1 Nuñez-Peralta, Claudia
A1 Llauger Rossello, Jaume
A1 Segovia, Sonia
A1 De Luna, Noemí
A1 Suarez-Calvet, Xavier
A1 Illa, Isabel
A1 Pompe Spanish Study group, 
A1 Díaz-Manera, Jordi
A1 Gallardo, Eduard
AB To analyze the microRNA profile in serum of patients with Adult Onset Pompe disease (AOPD). We analyzed the expression of 185 microRNAs in serum of 15 AOPD patients and five controls using microRNA PCR Panels. The expression levels of microRNAs that were deregulated were further studied in 35 AOPD patients and 10 controls using Real-Time PCR. Additionally, the skeletal muscle expression of microRNAs which showed significant increase levels in serum samples was also studied. Correlations between microRNA serum levels and muscle function test, spirometry, and quantitative muscle MRI were performed (these data correspond to the study NCT01914536 at ClinicalTrials.gov). We identified 14 microRNAs that showed different expression levels in serum samples of AOPD patients compared to controls. We validated these results in a larger cohort of patients and we found increased levels of three microRNAs, the so called dystromirs: miR-1-3p, miR-133a-3p, and miR-206. These microRNAs are involved in muscle regeneration and the expression of these was increased in patients' muscle biopsies. Significant correlations between microRNA levels and muscle function test were found. Serum expression levels of dystromirs may represent additional biomarkers for the follow-up of AOPD patients.
YR 2019
FD 2019-06-12
LK https://hdl.handle.net/10668/26472
UL https://hdl.handle.net/10668/26472
LA en
DS RISalud
RD Apr 18, 2025