RT Journal Article T1 Predicting dynamic response to neoadjuvant chemotherapy in breast cancer: a novel metabolomics approach. A1 Diaz, Caridad A1 Gonzalez-Olmedo, Carmen A1 Diaz-Beltran, Leticia A1 Camacho, Jose A1 Mena Garcia, Patricia A1 Martin-Blazquez, Ariadna A1 Fernandez-Navarro, Monica A1 Ortega-Granados, Ana Laura A1 Galvez-Montosa, Fernando A1 Marchal, Juan Antonio A1 Vicente, Francisca A1 Perez Del Palacio, Jose A1 Sanchez-Rovira, Pedro K1 ASCA K1 LC-HRMS K1 breast cancer K1 neoadjuvant chemotherapy K1 personalized medicine K1 treatment response AB Neoadjuvant chemotherapy (NACT) outcomes vary according to breast cancer (BC) subtype. Since pathologic complete response is one of the most important target endpoints of NACT, further investigation of NACT outcomes in BC is crucial. Thus, identifying sensitive and specific predictors of treatment response for each phenotype would enable early detection of chemoresistance and residual disease, decreasing exposures to ineffective therapies and enhancing overall survival rates. We used liquid chromatography-high-resolution mass spectrometry (LC-HRMS)-based untargeted metabolomics to detect molecular changes in plasma of three different BC subtypes following the same NACT regimen, with the aim of searching for potential predictors of response. The metabolomics data set was analyzed by combining univariate and multivariate statistical strategies. By using ANOVA-simultaneous component analysis (ASCA), we were able to determine the prognostic value of potential biomarker candidates of response to NACT in the triple-negative (TN) subtype. Higher concentrations of docosahexaenoic acid and secondary bile acids were found at basal and presurgery samples, respectively, in the responders group. In addition, the glycohyocholic and glycodeoxycholic acids were able to classify TN patients according to response to treatment and overall survival with an area under the curve model > 0.77. In relation to luminal B (LB) and HER2+ subjects, it should be noted that significant differences were related to time and individual factors. Specifically, tryptophan was identified to be decreased over time in HER2+ patients, whereas LysoPE (22:6) appeared to be increased, but could not be associated with response to NACT. Therefore, the combination of untargeted-based metabolomics along with longitudinal statistical approaches may represent a very useful tool for the improvement of treatment and in administering a more personalized BC follow-up in the clinical practice. PB John Wiley & Sons Ltd. YR 2022 FD 2022-03-24 LK http://hdl.handle.net/10668/19508 UL http://hdl.handle.net/10668/19508 LA en NO Díaz C, González-Olmedo C, Díaz-Beltrán L, Camacho J, Mena García P, Martín-Blázquez A, et al. Predicting dynamic response to neoadjuvant chemotherapy in breast cancer: a novel metabolomics approach. Mol Oncol. 2022 Jul;16(14):2658-2671. DS RISalud RD Feb 14, 2025