RT Journal Article
T1 Analysis of Menstrual Blood Stromal Cells Reveals SOX15 Triggers Oocyte-Based Human Cell Reprogramming
A1 Lopez-Caraballo, Lidia
A1 Martorell-Marugan, Jordi
A1 Carmona-Saez, Pedro
A1 Gonzalez-Muñoz, Elena
K1 Stromal cells
K1 Menstruation
K1 Blood cells
K1 Cellular reprogramming
K1 Oocytes
K1 Células del estroma
K1 Menstruación
K1 Células sanguíneas
K1 Reprogramación celular
K1 Oocitos
AB Cell reprogramming has revolutionized cell and regenerative biology field. However, human iPS derivation remains inefficient and variable. A better knowledge of molecular processes and the rationale underlying the importance of somatic cell origin is crucial to uncover reprogramming mechanisms. Here, we analyze the molecular profile of different human somatic cell types. We show menstrual blood-derived stromal cells (MnSCs) have a distinct, reprogramming prone, profile, and we identify SOX15 from their oocyte-related signature as a prominent responsible candidate. SOX15 orchestrates an efficient oocyte-based reprogramming combination when overexpressed with the also oocyte-enriched histone chaperone ASF1A and OCT4 and, through specific mechanism, generates iPSCs with distinguishable pluripotent state that further present higher differentiation capacity than canonical iPSCs. Our work supports the presence of different pluripotency states in reprogramming and the importance of using metaphase-II oocyte and MnSCs information to provide alternative reprogramming combinations and, importantly, to improve and understand pluripotency acquisition.
PB Cell Press, Elsevier
YR 2020
FD 2020-08-21
LK http://hdl.handle.net/10668/3545
UL http://hdl.handle.net/10668/3545
LA en
NO Lopez-Caraballo L, Martorell-Marugan J, Carmona-Saez P, Gonzalez-Muñoz E. Analysis of Menstrual Blood Stromal Cells Reveals SOX15 Triggers Oocyte-Based Human Cell Reprogramming. iScience. 2020 Aug 21;23(8):101376
DS RISalud
RD Apr 6, 2025