RT Journal Article T1 Evaluation of Phenolic Compounds and Pigments Content in Yellow Bell Pepper Wastes. A1 Razola-Diaz, Maria Del Carmen A1 Gomez-Caravaca, Ana Mª A1 Lopez de Andres, Julia A1 Voltes-Martinez, Ana A1 Zamora, Alberto A1 Perez-Molina, Gema M A1 Castro, David J A1 Marchal, Juan Antonio A1 Verardo, Vito K1 Capsicum annuum L. K1 HPLC-MS K1 antioxidant activity K1 flavonoids K1 phenolic acids K1 recovery of bioactive compounds AB Bell peppers are one of the most important species consumed and cultivated in Spain. Peppers are a source of carotenoids and phenolic compounds widely associated with biological activities such as antimicrobial, antiseptic, anticancer, counterirritant, cardioprotective, appetite stimulator, antioxidant, and immunomodulator. However, undersized and damaged fruits are usually wasted. Thus, in order to evaluate the phenolic content, a Box-Behnken design has been carried out to optimize the extraction from Capsicum annuum yellow pepper by ultrasound-assisted extraction (UAE). The independent factors were time (min), ethanol/water (% v/v) and solvent/sample ratio (v/w). The model was validated by ANOVA and confirmed. Furthermore, the whole pepper and the pepper without peduncles and seeds were extracted using optimal conditions and characterized by HPLC-ESI-TOF-MS. Moreover, their antioxidant activities, measured by three different methods (DPPH, ABTS, and FRAP), carotenoid composition, assessed by HPLC-MS, and chlorophyll content, assessed by a spectrophotometric method, were compared. A total of 38 polar compounds were found of which seven have been identified in pepper fruit extracts for the first time. According to the results, whole pepper (WP) samples presented higher content in phenolic acids; meanwhile, the edible portion (EP) was higher in flavonoids. No differences were found in the antioxidant activity except for the FRAP assay where the WP sample showed higher radical scavenging activity. EP samples showed the highest content of carotenoids and WP ones in chlorophylls. PB MDPI AG SN 2076-3921 YR 2022 FD 2022-03-10 LK http://hdl.handle.net/10668/20777 UL http://hdl.handle.net/10668/20777 LA en NO Razola-Díaz MDC, Gómez-Caravaca AM, López de Andrés J, Voltes-Martínez A, Zamora A, Pérez-Molina GM, et al. Evaluation of Phenolic Compounds and Pigments Content in Yellow Bell Pepper Wastes. Antioxidants (Basel). 2022 Mar 15;11(3):557. DS RISalud RD Apr 7, 2025