%0 Journal Article
%A Casuso, Rafael A
%A Al Fazazi, Saad
%A Plaza-Díaz, Julio
%A Ruiz-Ojeda, Francisco J
%A Rueda-Robles, Ascensión
%A Aragón-Vela, Jerónimo
%A Huertas, Jesús R
%T Physiological Doses of Hydroxytyrosol Modulate Gene Expression in Skeletal Muscle of Exercised Rats.
%D 2021
%@ 2075-1729
%U https://hdl.handle.net/10668/26192
%X We tested whether physiological doses of hydroxytyrosol (HT) may alter the mRNA transcription of key metabolic genes in exercised skeletal muscle. Two groups of exercise-trained Wistar rats, HTlow and HTmid, were supplemented with 0.31 and 4.61 mg/kg/d of HT, respectively, for 10 weeks. Another two groups of rats were not supplemented with HT; one remained sedentary and the other one was exercised. After the experimental period, the soleus muscle was removed for qRT-PCR and western blot analysis. The consumption of 4.61 mg/kg/d of HT during exercise increased the mRNA expression of important metabolic proteins. Specifically, 4.61 mg/kg/d of HT may upregulate long-chain fatty acid oxidation, lactate, and glucose oxidation as well as mitochondrial Krebs cycle in trained skeletal muscle. However, a 4.61 mg/kg/d of HT may alter protein translation, as in spite of the increment showed by CD36 and GLUT4 at the mRNA level this was not translated to higher protein content.
%K gene expression
%K glycolysis
%K mitochondria
%K polyphenols
%~