RT Journal Article
T1 Controlling the 3D architecture of Self-Lifting Auto-generated Tissue Equivalents (SLATEs) for optimized corneal graft composition and stability.
A1 Gouveia, Ricardo M
A1 González-Andrades, Elena
A1 Cardona, Juan C
A1 González-Gallardo, Carmen
A1 Ionescu, Ana M
A1 Garzon, Ingrid
A1 Alaminos, Miguel
A1 González-Andrades, Miguel
A1 Connon, Che J
K1 Tissue templating
K1 Corneal stroma
K1 SLATEs
K1 Animales
K1 Anisotropía
K1 Materiales biocompatibles
K1 Colágeno
K1 Córnea
K1 Sustancia propia
K1 Epitelio anterior
K1 Matriz extracelular
K1 Estudios de seguimiento
K1 Humanos
K1 Inflamación
K1 Elevación
K1 Péptidos
K1 Conejos
K1 Células del Estroma
AB Ideally, biomaterials designed to play specific physical and physiological roles in vivo should comprise components and microarchitectures analogous to those of the native tissues they intend to replace. For that, implantable biomaterials need to be carefully designed to have the correct structural and compositional properties, which consequently impart their bio-function. In this study, we showed that the control of such properties can be defined from the bottom-up, using smart surface templates to modulate the structure, composition, and bio-mechanics of human transplantable tissues. Using multi-functional peptide amphiphile-coated surfaces with different anisotropies, we were able to control the phenotype of corneal stromal cells and instruct them to fabricate self-lifting tissues that closely emulated the native stromal lamellae of the human cornea. The type and arrangement of the extracellular matrix comprising these corneal stromal Self-Lifting Analogous Tissue Equivalents (SLATEs) were then evaluated in detail, and was shown to correlate with tissue function. Specifically, SLATEs comprising aligned collagen fibrils were shown to be significantly thicker, denser, and more resistant to proteolytic degradation compared to SLATEs formed with randomly-oriented constituents. In addition, SLATEs were highly transparent while providing increased absorption to near-UV radiation. Importantly, corneal stromal SLATEs were capable of constituting tissues with a higher-order complexity, either by creating thicker tissues through stacking or by serving as substrate to support a fully-differentiated, stratified corneal epithelium. SLATEs were also deemed safe as implants in a rabbit corneal model, being capable of integrating with the surrounding host tissue without provoking inflammation, neo-vascularization, or any other signs of rejection after a 9-months follow-up. This work thus paves the way for the de novo bio-fabrication of easy-retrievable, scaffold-free human tissues with controlled structural, compositional, and functional properties to replace corneal, as well as other, tissues.
PB Elsevier
SN 0142-9612
YR 2017
FD 2017-03
LK http://hdl.handle.net/10668/2608
UL http://hdl.handle.net/10668/2608
LA en
NO Gouveia RM, González-Andrades E, Cardona JC, González-Gallardo C, Ionescu AM, Garzon I, et al. Controlling the 3D architecture of Self-Lifting Auto-generated Tissue Equivalents (SLATEs) for optimized corneal graft composition and stability. Biomaterials. 2017                         ; 121:205-219
NO Journal Article;
DS RISalud
RD Apr 10, 2025