RT Journal Article
T1 Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus.
A1 Domingo-Reines, Joan
A1 Martinez-Navajas, Gonzalo
A1 Montes, Rosa
A1 Lamolda, Mar
A1 Simon, Iris
A1 Castaño, Julio
A1 Rios-Pelegrina, Rosa
A1 Lopez-Hidalgo, Javier Luis
A1 Garcia Del Moral, Raimundo
A1 Marchal, Juan A
A1 Real, Pedro J
A1 Ramos-Mejia, Veronica
K1 acute myeliod leukemia
K1 disease model
K1 hematopoieisis
K1 human stem cell
K1 pediatric
AB Pediatric acute myeloid leukemia (AML) is a rare and heterogeneous disease that remains the major cause of mortality in children with leukemia. To improve the outcome of pediatric AML we need to gain knowledge on the biological bases of this disease. NUP98-KDM5A (NK5A) fusion protein is present in a particular subgroup of young pediatric patients with poor outcome. We report the generation and characterization of human Embryonic Stem Cell (hESC) clonal lines with inducible expression of NK5A. Temporal control of NK5A expression during hematopoietic differentiation from hESC will be critical for elucidating its participation during the leukemogenic process.
PB Frontiers Research Foundation
SN 2296-634X
YR 2022
FD 2022-05-12
LK http://hdl.handle.net/10668/20532
UL http://hdl.handle.net/10668/20532
LA en
NO Domingo-Reinés J, Martínez-Navajas G, Montes R, Lamolda M, Simón I, Castaño J, et al. Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus. Front Cell Dev Biol. 2022 Jun 1;10:846092.
DS RISalud
RD Mar 14, 2025