RT Journal Article
T1 Penicillin susceptibility among invasive MSSA infections: a multicentre study in 16 Spanish hospitals.
A1 Mama, Olouwafemi Mistourath
A1 Aspiroz, Carmen
A1 Lozano, Carmen
A1 Ruiz-Ripa, Laura
A1 Azcona, José Manuel
A1 Seral, Cristina
A1 Cercenado, Emilia
A1 López-Cerero, Lorena
A1 Palacian, Pilar
A1 Belles-Belles, Alba
A1 Berdonces, Pilar
A1 Siller, María
A1 Aguirre-Quiñonero, Amaia
A1 Zarazaga, Myriam
A1 Torres, Carmen
A1 Spanish Study Group of Clinical S. aureus CC398, 
AB To determine the prevalence of penicillin susceptibility among MSSA causing bloodstream infections (BSIs) in 16 Spanish hospitals and to characterize the penicillin-susceptible MSSA (MSSA-PENS) isolates. A total of 1011 Staphylococcus aureus isolates were collected from blood cultures in 16 Spanish hospitals during 2018-19 (6-12 months) and their susceptibility to 18 antimicrobials was determined. The MSSA-PENS isolates were selected and examined by PCR to determine the presence of the blaZ gene, other resistance genes and the genes lukF/lukS-PV, eta, etb and tst. The immune evasion cluster (IEC) type was also analysed. All the MSSA-PENS isolates were submitted to S. aureus protein A (spa) typing and the clonal complexes (CCs) were assigned according to their spa type. The prevalence of MSSA was 74.6% (754/1011) and 14.9% (151/1011) were MSSA-PENS-blaZnegative. MSSA-PENS-blaZnegative isolates (n = 151) were ascribed to 88 spa types and 11 CCs. The most frequent CCs were CC5 (35/151) and CC398 (25/151), with t002-CC5 and t571-CC398 being the most common lineages. Pan-susceptibility was identified in 117 of the 151 MSSA-PENS-blaZnegative isolates (77.5%). In the remaining isolates, erythromycin and clindamycin resistance was the most frequent resistance found, although tobramycin, ciprofloxacin, fusidic acid, mupirocin and/or tetracycline resistance was also detected. Thirty-eight MSSA-PENS-blaZnegative isolates were IEC negative and four isolates were Panton-Valentine leucocidin ('PVL') positive. A high penicillin susceptibility rate was detected among MSSA, opening therapeutic opportunities for BSIs. The emergence of new successful MSSA-PENS clones could be responsible for these data. The detection among MSSA-PENS-blaZnegative isolates of the clonal lineage CC398 or the absence of an IEC raises questions about their possible animal origin, requiring further analysis.
YR 2021
FD 2021
LK https://hdl.handle.net/10668/27189
UL https://hdl.handle.net/10668/27189
LA en
DS RISalud
RD Apr 12, 2025