RT Journal Article
T1 Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms.
A1 Henn, Dominic
A1 Bandner-Risch, Doris
A1 Perttunen, Hilja
A1 Schmied, Wolfram
A1 Porras, Carlos
A1 Ceballos, Francisco
A1 RodrÍguez-Losada, Noela
A1 Schäfers, Hans-Joachim
K1 Aneurisma de la Aorta
K1 Enfermedades de las Válvulas Cardíacas
K1 Hipertensión
K1 Adulto
K1 Estudios de Asociación Genética
K1 Expresión Génica
K1 Tejidos
K1 Humanos
K1 Factor 2B Eucariótico de Iniciación
K1 Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
K1 Femenino
K1 Masculino
AB Hypertension and congenital aortic valve malformations are frequent causes of ascending aortic aneurysms. The molecular mechanisms of aneurysm formation under these circumstances are not well understood. Reference genes for gene activity studies in aortic tissue that are not influenced by aortic valve morphology and its hemodynamic consequences, aortic dilatation, hypertension, or antihypertensive medication are not available so far. This study determines genes in ascending aortic tissue that are independent of these parameters. Tissue specimens from dilated and undilated ascending aortas were obtained from 60 patients (age ≤70 years) with different morphologies of the aortic valve (tricuspid undilated n = 24, dilated n = 11; bicuspid undilated n = 6, dilated n = 15; unicuspid dilated n = 4). Of the studied individuals, 36 had hypertension, and 31 received ACE inhibitors or AT1 receptor antagonists. The specimens were obtained intraoperatively from the wall of the ascending aorta. We analyzed the expression levels of 32 candidate reference genes by quantitative RT-PCR (RT-qPCR). Differential expression levels were assessed by parametric statistics. The expression analysis of these 32 genes by RT-qPCR showed that EIF2B1, ELF1, and PPIA remained constant in their expression levels in the different specimen groups, thus being insensitive to aortic valve morphology, aortic dilatation, hypertension, and medication with ACE inhibitors or AT1 receptor antagonists. Unlike many other commonly used reference genes, the genes EIF2B1, ELF1, and PPIA are neither confounded by aortic comorbidities nor by antihypertensive medication and therefore are most suitable for gene expression analysis of ascending aortic tissue.
PB Public Library of Science
YR 2013
FD 2013-01-11
LK http://hdl.handle.net/10668/877
UL http://hdl.handle.net/10668/877
LA en
NO Henn D, Bandner-Risch D, Perttunen H, Schmied W, Porras C, Ceballos F, et al. Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms. PLoS ONE. 2013; 8(1):e54132
NO Journal Article;
DS RISalud
RD Apr 15, 2025