RT Journal Article
T1 Proteomic Analysis of the Secretome and Exosomes of Feline Adipose-Derived Mesenchymal Stem Cells
A1 Villatoro, Antonio J.
A1 Martín-Astorga, María del Carmen
A1 Alcoholado, Cristina
A1 Sánchez-Martín, María del Mar
A1 Becerra, José
K1 Exosomes
K1 Feline
K1 Mesenchymal stem cells
K1 Secretome
K1 Ultrahigh-performance liquid chromatography-high-resolution mass spectrometry (UHPLC–HRMS).
K1 Exosomas
K1 Calicivirus felino
K1 Células madre mesenquimatosas
AB Mesenchymal stem cells (MSCs) have been shown to have therapeutic efficacy in different complex pathologies in feline species. This effect is attributed to the secretion of a wide variety of bioactive molecules and extracellular vesicles, such as exosomes, with significant paracrine activity, encompassed under the concept of the secretome. However, at present, the exosomes from feline MSCs have not yet been studied in detail. The objective of this study is to analyze and compare the protein profiles of the secretome as a whole and its exosomal fraction from feline adipose-derived MSCs (fAd-MSCs). For this, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Protein-Protein Interaction Networks Functional Enrichment Analysis (STRING) were utilized. A total of 239 proteins were identified in the secretome, and 228 proteins specific to exosomes were identified, with a total of 133 common proteins. The proteins identified in the secretome were located in the extracellular regions and in the cytoplasm, while the exosomal proteins were located mainly in the membrane, cytoplasm and cytosol. Regarding function, in the secretome, proteins involved in different metabolic pathways, in pathways related to the immune system and the endocrine system and in the processing of proteins in the endoplasmic reticulum predominated. In contrast, proteins specific to exosomes were predominantly associated with endocytosis, cell junctions, platelet activation and other cell signaling pathways. The possible future use of the secretome, or some of its components, such as exosomes, would provide a non-cell-based therapeutic strategy for the treatment of different diseases that would avoid the drawbacks of cell therapy.
PB MDPI AG
YR 2021
FD 2021-01-24
LK http://hdl.handle.net/10668/3476
UL http://hdl.handle.net/10668/3476
LA en
NO Villatoro AJ; Martín-Astorga MdC; Alcoholado, C; Sánchez-Martín MdM; Becerra J. Proteomic Analysis of the Secretome and Exosomes of Feline Adipose-Derived Mesenchymal Stem Cells. Animals 2021, 11, 295.
DS RISalud
RD Apr 17, 2025