RT Journal Article
T1 Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture.
A1 Murtazina, Alima
A1 Ruiz Alcala, Gloria
A1 Jimenez-Martinez, Yaiza
A1 Marchal, Juan Antonio
A1 Tarabayeva, Anel
A1 Bitanova, Elmira
A1 McDougall, Gordon
A1 Bishimbayeva, Nazira
A1 Boulaiz, Houria
K1 apoptosis
K1 colon cancer cells
K1 cytochrome c
K1 differentiation
K1 electron microscopy
K1 inhibition of proliferation
K1 plant polysaccharides
K1 wheat cell culture
AB There is a global need to discover effective anti-cancerous compounds from natural sources. Cultivated wheat cells can be a valuable source of non-toxic or low toxic plant-derived polysaccharides. In this study, we evaluated the anti-cancer ability of seven fractions of wheat cell culture polysaccharides (WCCPSs) in the HCT-116 colon cancer cell line. Almost all (6/7) fractions had an inhibitory effect on the proliferation of colon cancer cells, and two fractions (A-b and A-f) had considerable therapeutic indexes. The WCCPS fractions induced cell cycle arrest in the G1 phase and induced different rates of apoptosis (≤48%). Transmission and scanning electron microscopy revealed that WCCPS fractions caused apoptotic changes in the nucleus and cytoplasm, including damage to mitochondria and external morphological signs of apoptosis. In addition, the WCCPSs induced an increase in the levels of Bax, cytochrome c, and caspases 8 and 3, indicating that cell death progressed through intrinsic and extrinsic pathways of apoptosis. Furthermore, some fractions caused a significant decrease of c-Myc, b-catenin, NFkB2, and HCAM (CD 44) levels, indicating enhanced cell differentiation. Thus, for the first time, our results provide a proof of concept of the anti-cancer capacity of WCCPS fractions in colorectal cancer.
PB MDPI AG
SN 1999-4923
YR 2022
FD 2022-05-17
LK http://hdl.handle.net/10668/21549
UL http://hdl.handle.net/10668/21549
LA en
NO Murtazina A, Ruiz Alcala G, Jimenez-Martinez Y, Marchal JA, Tarabayeva A, Bitanova E, et al. Potential of Polysaccharides Derived from Wheat Cell Culture. Pharmaceutics. 2022 May 20;14(5):1100.
DS RISalud
RD Apr 17, 2025