RT Generic
T1 Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review
A1 Ruiz-Ojeda, Francisco Javier
A1 Iris Ruperez, Azahara
A1 Gomez-Llorente, Carolina
A1 Gil, Angel
A1 Maria Aguilera, Concepcion
K1 adipocytes
K1 beige cells
K1 brown adipose tissue
K1 cell culture techniques
K1 cell differentiation
K1 in vitro techniques
K1 obesity
K1 white adipose tissue
K1 Brown adipose-tissue
K1 Inhibits adipocyte differentiation
K1 Mesenchymal stem-cells
K1 Morphogenetic protein 4
K1 Human pre-adipocytes
K1 Human preadipocytes
K1 White fat
K1 Porcine preadipocytes
K1 Gene-expression
K1 3t3-l1 cells
AB Over the last several years, the increasing prevalence of obesity has favored an intense study of adipose tissue biology and the precise mechanisms involved in adipocyte differentiation and adipogenesis. Adipocyte commitment and differentiation are complex processes, which can be investigated thanks to the development of diverse in vitro cell models and molecular biology techniques that allow for a better understanding of adipogenesis and adipocyte dysfunction associated with obesity. The aim of the present work was to update the different animal and human cell culture models available for studying the in vitro adipogenic differentiation process related to obesity and its co-morbidities. The main characteristics, new protocols, and applications of the cell models used to study the adipogenesis in the last five years have been extensively revised. Moreover, we depict co-cultures and three-dimensional cultures, given their utility to understand the connections between adipocytes and their surrounding cells in adipose tissue.
PB MDPI AG
YR 2016
FD 2016-06-24
LK http://hdl.handle.net/10668/19267
UL http://hdl.handle.net/10668/19267
LA en
NO Ruiz-Ojeda FJ, Rupérez AI, Gomez-Llorente C, Gil A, Aguilera CM. Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review. Int J Mol Sci. 2016 Jun 30;17(7):1040.
NO This work was supported by Junta de Andalucía (project number CTS-6770; Secretaría General de Universidades, Investigación y Tecnología. Consejería de Economía, Innovación y Ciencia. Implicaciones biológicas de genes de las vías de señalización de la insulina, inflamación y de la matriz extracelular en cultivos de células madre mesenquimales de tejido adiposo humano). Francisco JavierRuiz-Ojedawas funded by a Formación de Profesorado Universitario (FPU) stipend from the Ministry of Education and Science of the Spanish Government (AP2012-02068). This paper will be part of Francisco Javier Ruiz-Ojeda’s doctorate, which is being completed as part of the “Nutrition and Food Sciences Program” at the University of Granada, Spain.
DS RISalud
RD Apr 11, 2025