RT Journal Article
T1 Enterocin Cross-Resistance Mediated by ABC Transport Systems
A1 Teso-Pérez, Claudia
A1 Martínez-Bueno, Manuel
A1 Peralta-Sánchez, Juan Manuel
A1 Valdivia, Eva
A1 Maqueda, Mercedes
A1 Fárez-Vidal, M Esther
A1 Martín-Platero, Antonio M.
K1 Enterocin
K1 ABC transporter
K1 Bacteriocin immunity
K1 MR10A/B
K1 AS-48
K1 Transportadoras de casetes de unión a ATP
K1 Bacteriocinas
K1 Inmunidad
K1 Enterococcus faecalis
K1 Vectores genéticos
AB In their struggle for life, bacteria frequently produce antagonistic substances against competitors. Antimicrobial peptides produced by bacteria (known as bacteriocins) are active against other bacteria, but harmless to their producer due to an associated immunity gene that prevents self-inhibition. However, knowledge of cross-resistance between different types of bacteriocin producer remains very limited. The immune function of certain bacteriocins produced by the Enterococcus genus (known as enterocins) is mediated by an ABC transporter. This is the case for enterocin AS-48, a gene cluster that includes two ABC transporter-like systems (Transporter-1 and 2) and an immunity protein. Transporter-2 in this cluster shows a high similarity to the ABC transporter-like system in MR10A and MR10B enterocin gene clusters. The aim of our study was to determine the possible role of this ABC transporter in cross-resistance between these two different types of enterocin. To this end, we designed different mutants (Tn5 derivative and deletion mutants) of the as-48 gene cluster in Enterococcus faecalis and cloned them into the pAM401 shuttle vector. Antimicrobial activity assays showed that enterocin AS-48 Transporter-2 is responsible for cross-resistance between AS-48 and MR10A/B enterocin producers and allowed identification of the MR10A/B immunity gene system. These findings open the way to the investigation of resistance beyond homologous bacteriocins.
PB MDPI
YR 2021
FD 2021-06-30
LK http://hdl.handle.net/10668/4205
UL http://hdl.handle.net/10668/4205
LA en
NO Teso-Pérez C, Martínez-Bueno M, Peralta-Sánchez JM, Valdivia E, Maqueda M, Fárez-Vidal ME, et al. Enterocin Cross-Resistance Mediated by ABC Transport Systems. Microorganisms. 2021 Jun 30;9(7):1411
DS RISalud
RD Apr 7, 2025