RT Journal Article
T1 Superficial Characteristics and Functionalization Effectiveness of Non-Toxic Glutathione-Capped Magnetic, Fluorescent, Metallic and Hybrid Nanoparticles for Biomedical Applications
A1 Fernandez-Ponce, C.
A1 Manuel, J. M.
A1 Fernandez-Cisnal, R.
A1 Felix, E.
A1 Beato-Lopez, J.
A1 Munoz-Miranda, J. P.
A1 Beltran, A. M.
A1 Santos, A. J.
A1 Morales, F. M.
A1 Yeste, M. P.
A1 Bomati-Miguel, O.
A1 Litran, R.
A1 Garcia-Cozar, F.
K1 Functionalization
K1 Glutathione
K1 Surface modifications
K1 Colloidal stability
K1 Cytotoxicity
K1 Electron microscopy
K1 Magnetic nanoparticle
K1 Gold nanoparticle
K1 Quantum dot
AB An optimal design of nanoparticles suitable for biomedical applications requires proper functionalization, a key step in the synthesis of such nanoparticles, not only for subsequent crosslinking to biological targets and to avoid cytotoxicity, but also to endow these materials with colloidal stability. In this sense, a reliable characterization of the effectiveness of the functionalization process would, therefore, be crucial for subsequent bioconjugations. In this work, we have analyzed glutathione as a means to functionalize four of the most widely used nanoparticles in biomedicine, one of which is a hybrid gold-magnetic-iron-oxide nanoparticle synthetized by a simple and novel method that we propose in this article. We have analyzed the colloidal characteristics that the glutathione capping provides to the different nanoparticles and, using information on the Z-potential, we have deduced the chemical group used by glutathione to link to the nanoparticle core. We have used electron microscopy for further structural and chemical characterization of the nanoparticles. Finally, we have evaluated nanoparticle cytotoxicity, studying cell viability after incubation with different concentrations of nanoparticles, showing their suitability for biomedical applications.
PB MDPI
YR 2021
FD 2021-02-22
LK http://hdl.handle.net/10668/19303
UL http://hdl.handle.net/10668/19303
LA en
NO Fernández-Ponce C, Mánuel JM, Fernández-Cisnal R, Félix E, Beato-López J, Muñoz-Miranda JP, et al. Superficial Characteristics and Functionalization Effectiveness of Non-Toxic Glutathione-Capped Magnetic, Fluorescent, Metallic and Hybrid Nanoparticles for Biomedical Applications. Metals [Internet]. 26 de febrero de 2021;11(3):383
DS RISalud
RD Apr 10, 2025