RT Journal Article T1 Confirmed presence of aedes (rusticoidus) refiki Medschid, 1928 in a continental dry Mediterranean peri-urban environment in south-central Spain A1 Casades-Marti, Laia A1 Frias, Mario A1 Delacour, Sarah A1 Ruiz-Fons, Francisco K1 Aedes K1 Barcoding K1 Mediterranean K1 Peri-urban K1 Scarce K1 Vector competence K1 Albopictus K1 Aegypti AB Background The 'snow-melt mosquito' aedes (rusticoidus) refiki is a rare species with a wide distribution in Europe that is usually defined as an aggressive mosquito for mammals, including humans. During a mosquito survey in a peri-urban area in south-central mainland Spain, adult Ae. refiki females were captured and identified by morphological traits. The presence of this species of mosquito has never been molecularly confirmed under continental dry Mediterranean climatic influence with scarce number of days with snow on soil. The aim of this study was to confirm by amplification and sequencing of mitochondrial cytochrome c oxidase subunit I (COI) and internal transcribed spacer 2 (ITS2) region. Results We also successfully amplified and typed the species molecularly by COI and ITS2 regions. The peri-urban area where Ae. refiki was found contrasts with the reported cold, humid and snowy environments required by the species to breed. Conclusions This finding suggests that the species is already adapted to continental dry Mediterranean environments, questioning whether it is a truly stenotopic species of cold snowy environments. PB BioMed Central YR 2022 FD 2022-04-19 LK http://hdl.handle.net/10668/20379 UL http://hdl.handle.net/10668/20379 LA en NO Casades-Martí L, Frías M, Delacour S, Ruiz-Fons F. Confirmed presence of aedes (rusticoidus) refiki Medschid, 1928 in a continental dry Mediterranean peri-urban environment in south-central Spain. BMC Zool. 2022 Apr 29;7(1):21 NO Casades-Martí L, Frías M, Delacour S, Ruiz-Fons F. Confirmed presence of aedes (rusticoidus) refiki Medschid, 1928 in a continental dry Mediterranean peri-urban environment in south-central Spain. BMC Zool. 2022 Apr 29;7(1):21 DS RISalud RD Apr 12, 2025