%0 Journal Article
%A Vivancos, Ana
%A Aranda, Enrique
%A Benavides, Manuel
%A Elez, Elena
%A Gomez-España, Maria Auxiliadora
%A Toledano, Marta
%A Alvarez, Martina
%A Parrado, Maria Rosario Chica
%A Garcia-Barberan, Vanesa
%A Diaz-Rubio, Eduardo
%T Comparison of the Clinical Sensitivity of the Idylla Platform and the OncoBEAM RAS CRC Assay for KRAS Mutation Detection in Liquid Biopsy Samples.
%D 2019
%U http://hdl.handle.net/10668/14152
%X KRAS mutations are common in colorectal cancer (CRC). In this setting, mutation status determination in circulating-free DNA from blood samples (liquid biopsy) has been shown to be a viable alternative to tissue testing. The objective of this study was to compare the sensitivity of two liquid biopsy methods for detecting KRAS mutations in plasma samples from metastatic CRC patients. Samples with a positive (KRAS-MUT+) result and a mutant allelic fraction (MAF) )< 5% according to theOncoBEAM RAS CRC assay were pairly analyzed by the Idylla ctKRAS Mutation Test (n= 116). In a cohort of 71 patients with atleast 1 year of follow-up,the progression-free survival (PFS) was determined according to MAF values. Idylla detected KRAS mutations in 81/116 OncoBEAM KRAS-MUT+ samples with MAF< 5% and in 48/79 samples with MAF < 1%. Concordance betweenOncoBEAM and Idylla signifcantly improved at higher MAF values. PFS rates at 6 and 12 months tended to be lower in patients with MAF levelsbetween 1% and 5% than in those with levels <1%. OncoBEAM demonstrated greater sensitivity for plasma detection of KRAS mutations than Idylla. Importantly, our data identifed a “gray zone” below 1% MAF where Idylla showed reduced KRAS mutation detection, highlighting the importance of an accurate method to provide the mutational status of CRC patients.
%K Middle Aged
%K Mutation
%K Prognosis
%K Proto-Oncogene Proteins p21(ras)
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