TY - JOUR AU - Gutiérrez, Gabriel AU - Millán-Zambrano, Gonzalo AU - Medina, Daniel A AU - Jordán-Pla, Antonio AU - Pérez-Ortín, José E AU - Peñate, Xenia AU - Chávez, Sebastián PY - 2017 DO - 10.1186/s13072-017-0165-x UR - http://hdl.handle.net/10668/11882 T2 - Epigenetics & chromatin AB - TFIIS stimulates RNA cleavage by RNA polymerase II and promotes the resolution of backtracking events. TFIIS acts in the chromatin context, but its contribution to the chromatin landscape has not yet been investigated. Co-transcriptional chromatin... LA - en KW - MNase-sensitive nucleosomes KW - MNase-seq KW - Nucleosomal fuzziness KW - Nucleosome mapping KW - TFIIS KW - High-Throughput Nucleotide Sequencing KW - Micrococcal Nuclease KW - Nucleosomes KW - Real-Time Polymerase Chain Reaction KW - Saccharomyces cerevisiae KW - Subtraction Technique KW - Transcriptional Elongation Factors TI - Subtracting the sequence bias from partially digested MNase-seq data reveals a general contribution of TFIIS to nucleosome positioning. TY - research article VL - 10 ER -