RT Journal Article
T1 Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering.
A1 Olmo, Alberto
A1 Yuste, Yaiza
A1 Serrano, Juan Alfonso
A1 Maldonado-Jacobi, Andres
A1 Pérez, Pablo
A1 Huertas, Gloria
A1 Pereira, Sheila
A1 Yufera, Alberto
A1 de la Portilla, Fernando
K1 electrical modeling
K1 impedance spectroscopy
K1 oscillation-based test
K1 skeletal muscle
K1 stem cell differentiation
AB In tissue engineering, of utmost importance is the control of tissue formation, in order to form tissue constructs of clinical relevance. In this work, we present the use of an impedance spectroscopy technique for the real-time measurement of the dielectric properties of skeletal myoblast cell cultures. The processes involved in the growth and differentiation of these cell cultures in skeletal muscle are studied. A circuit based on the oscillation-based test technique was used, avoiding the use of high-performance circuitry or external input signals. The effect of electrical pulse stimulation applied to cell cultures was also studied. The technique proved useful for monitoring in real-time the processes of cell growth and estimating the fill factor of muscular stem cells. Impedance spectroscopy was also useful to study the real-time monitoring of cell differentiation, obtaining different oscillation amplitude levels for differentiated and undifferentiated cell cultures. Finally, an electrical model was implemented to better understand the physical properties of the cell culture and control the tissue formation process.
YR 2020
FD 2020-06-02
LK http://hdl.handle.net/10668/15681
UL http://hdl.handle.net/10668/15681
LA en
DS RISalud
RD Apr 7, 2025