RT Journal Article
T1 Dermatophagoides pteronyssinus immunotherapy changes the T-regulatory cell activity.
A1 Gonzalez, M
A1 Doña, I
A1 Palomares, F
A1 Campo, P
A1 Rodriguez, M J
A1 Rondon, C
A1 Gomez, F
A1 Fernandez, T D
A1 Perkins, J R
A1 Escribese, M M
A1 Torres, M J
A1 Mayorga, C
K1 Antigens, Dermatophagoides
K1 Cells, Cultured
K1 Coculture Techniques
K1 Dermatophagoides pteronyssinus
AB Subcutaneous specific immunotherapy (SCIT) has been shown to modify the Dermatophagoides pteronissinus (DP) allergic response, characterized by generation of Treg cells. However, studies have reported no changes in the proportion of Treg cells after immunotherapy, indicating that the effects may be due to modifications in their regulatory activities. We aimed to determine whether Tregs generated by DP-SCIT can switch the allergic response to tolerant and study the involvement of suppressive cytokines on it. Twenty-four DP-allergic rhinitis patients were recruited, 16 treated with DP-SCIT and 8 untreated. Treg and T effector cells were isolated before and after DP-SCIT, and cocultured in different combinations with α-IL-10, α-TGF-β blocking antibodies and nDer p 1. Treg cells after DP-SCIT increased Th1 and decreased Th2 and Th9 proliferation. Similarly, they increased IL-10 and decreased IL-4 and IL-9-producing cells. α-IL-10 affected the activity of Treg cells obtained after DP-SCIT only. Finally, DP-specific IgG4 levels, Treg percentage and IL-10 production were correlated after DP-SCIT. These results demonstrate that DP-SCIT induces Treg cells with different suppressive activities. These changes could be mediated by IL-10 production and appear to play an important role in the induction of the tolerance response leading to a clinical improvement of symptoms.
PB Nature Publishing Group
YR 2017
FD 2017-09-20
LK http://hdl.handle.net/10668/11598
UL http://hdl.handle.net/10668/11598
LA en
NO Gonzalez M, Doña I, Palomares F, Campo P, Rodriguez MJ, Rondon C, et al. Dermatophagoides pteronyssinus immunotherapy changes the T-regulatory cell activity. Sci Rep. 2017 Sep 20;7(1):11949
NO The study was funded by grants from the Institute of Health “Carlos III” of the Ministry of Economy and Competitiveness: PI12/02481, PI15/02256 and PI15/00559, RETICS ARADyAL (RD16/0006/0001; RD16/0006/0015), Juan Rodes Program (JR15/00036) and Sara Borrell Program (CD14/00242), Salud 2000 project, SEAIC Foundation, PI-0542-2010, Andalusian Regional Ministry of Economy and Knowledge (CTS-7433), Andalusian Regional Ministry of Health, Nicolas Monardes Program (C-0044-2012 SAS2013). Grants were co-funded by the European Regional Development Fund (ERDF).
DS RISalud
RD Apr 18, 2025