RT Journal Article
T1 Combining data acquisition modes in liquid-chromatography-tandem mass spectrometry for comprehensive determination of acylcarnitines in human serum.
A1 Luque-Cordoba, D
A1 Calderon-Santiago, M
A1 Priego-Capote, F
K1 Acylcarnitines
K1 Data-dependent acquisition
K1 Data-independent acquisition
K1 Multiple reaction monitoring
K1 SPE–LC–MS/MS
K1 Serum
AB Acylcarnitines (ACs) are metabolites involved in fatty acid β-oxidation and organic acid metabolism. Metabolic disorders associated to these two processes can be evaluated by determining the complete profile of ACs. In this research, we present an overall strategy for identification, confirmation, and quantitative determination of acylcarnitines in human serum. By this strategy we identified the presence of 47 ACs from C2 to C24 with detection of the unsaturation degree by application of a data-independent acquisition (DIA) liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. Complementary, quantitative determination of ACs is based on a high-throughput and fully automated method consisting of solid-phase extraction on-line coupled to LC-MS/MS in data-dependent acquisition (DDA) to improve analytical features avoiding the errors associated to sample processing. Quantitation limits were at pg mL-1 level, the intra-day and between-day variability were below 15-20%, respectively; and the accuracy, expressed as bias, was always within ± 25%. The proposed method was tested with 40 human volunteers to determine the relative concentration of ACs in serum and identify predominant forms. Significant differences were detected by comparing the ACs profile of obese versus non-obese individuals.
PB Springer
YR 2022
FD 2022-06-27
LK http://hdl.handle.net/10668/20797
UL http://hdl.handle.net/10668/20797
LA en
NO Luque-Córdoba D, Calderón-Santiago M, Priego-Capote F. Combining data acquisition modes in liquid-chromatography-tandem mass spectrometry for comprehensive determination of acylcarnitines in human serum. Metabolomics. 2022 Jul 21;18(8):59
DS RISalud
RD Apr 6, 2025