RT Journal Article
T1 Oxidized LDL Increase the Proinflammatory Profile of Human Visceral Adipocytes Produced by Hypoxia
A1 Santiago-Fernández, Concepción
A1 Martín-Reyes, Flores
A1 Tome, Monica
A1 Gutierrez-Repiso, Carolina
A1 Fernandez-Garcia, Diego
A1 Ocaña-Wilhelmi, Luis
A1 Rivas-Becerra, Jose
A1 Tatzber, Franz
A1 Pursch, Edith
A1 Tinahones, Francisco J.
A1 García-Fuentes, Eduardo
A1 Garrido-Sánchez, Lourdes
K1 Hypoxia
K1 Scavenger receptors
K1 Oxidized LDL
K1 Adipocytes
K1 Morbid obesity
K1 Hipoxia
K1 Receptores depuradores
K1 Receptores de LDL oxidadas
K1 Adipocitos
K1 Obesidad mórbida
AB Background: Little is known about the effects of hypoxia on scavenger receptors (SRs) levels in adipocytes. We analyzed the effect of morbid obesity and hypoxia on SRs and inflammation markers in human visceral adipocytes and whether ox-LDL modify the inflammatory profile produced by hypoxia. Methods: We studied in 17 non-obese and 20 subjects with morbid obesity (MO) the mRNA expression of HIF-1α, SRs (LOX-1, MSR1, CL-P1 and CXCL16), IL6 and TNFα in visceral adipocytes and the effect of hypoxia with or without ox-LDL on visceral in vitro-differentiated adipocytes (VDA). Results: HIF-1α, TNFα, IL6, LOX-1, MSR1 and CXCL16 expression in adipocytes was increased in MO when compared with those in non-obese subjects (p < 0.05). The expression of most of the inflammatory markers and SRs gene correlated with HIF-1α. In VDA, hypoxia increased TNFα, IL6, MSR1, CXCL16 and CL-P1 (p < 0.05) in non-obese subjects, and TNFα, IL6, MSR1 and CXCL16 (p < 0.05) in MO. Silencing HIF-1α prevented the increase of TNFα, IL6, LOX-1, MSR1, CL-P1 and CXCL16 expression (p < 0.05). The combination of hypoxia and ox-LDL produced higher TNFα expression (p = 0.041). Conclusions: Morbid obesity and hypoxia increased SRs and inflammatory markers in visceral adipocytes. In a hypoxic state, ox-LDL increased the proinflammatory response of visceral adipocytes to hypoxia.
PB MDPI
YR 2021
FD 2021-11-18
LK http://hdl.handle.net/10668/4456
UL http://hdl.handle.net/10668/4456
LA en
NO Santiago-Fernández C, Martín-Reyes F, Tome M, Gutierrez-Repiso C, Fernandez-Garcia D, Ocaña-Wilhelmi L, et al. Oxidized LDL Increase the Proinflammatory Profile of Human Visceral Adipocytes Produced by Hypoxia. Biomedicines. 2021 Nov 18;9(11):1715
DS RISalud
RD Apr 8, 2025