RT Journal Article
T1 Evaluation of Fibrin-Agarose Tissue-Like Hydrogels Biocompatibility for Tissue Engineering Applications.
A1 Campos, Fernando
A1 Bonhome-Espinosa, Ana Belen
A1 Chato-Astrain, Jesús
A1 Sánchez-Porras, David
A1 García-García, Óscar Darío
A1 Carmona, Ramón
A1 López-López, Modesto T
A1 Alaminos, Miguel
A1 Carriel, Víctor
A1 Rodriguez, Ismael A
K1 biodegradation
K1 blood and biochemical profile
K1 fibrin-agarose hydrogels
K1 histological assessment
K1 in vivo biocompatibility
K1 tissue engineering
AB Generation of biocompatible and biomimetic tissue-like biomaterials is crucial to ensure the success of engineered substitutes in tissue repair. Natural biomaterials able to mimic the structure and composition of native extracellular matrices typically show better results than synthetic biomaterials. The aim of this study was to perform an in vivo time-course biocompatibility analysis of fibrin-agarose tissue-like hydrogels at the histological, imagenological, hematological, and biochemical levels. Tissue-like hydrogels were produced by a controlled biofabrication process allowing the generation of biomechanically and structurally stable hydrogels. The hydrogels were implanted subcutaneously in 25 male Wistar rats and evaluated after 1, 5, 9, and 12 weeks of in vivo follow-up. At each period of time, animals were analyzed using magnetic resonance imaging (MRI), hematological analyses, and histology of the local area in which the biomaterials were implanted, along with major vital organs (liver, kidney, spleen, and regional lymph nodes). MRI results showed no local or distal alterations during the whole study period. Hematology and biochemistry showed some fluctuation in blood cells values and in some biochemical markers over the time. However, these parameters were progressively normalized in the framework of the homeostasis process. Histological, histochemical, and ultrastructural analyses showed that implantation of fibrin-agarose scaffolds was followed by a progressive process of cell invasion, synthesis of components of the extracellular matrix (mainly, collagen) and neovascularization. Implanted biomaterials were successfully biodegraded and biointegrated at 12 weeks without any associated histopathological alteration in the implanted zone or distal vital organs. In summary, our in vivo study suggests that fibrin-agarose tissue-like hydrogels could have potential clinical usefulness in engineering applications in terms of biosafety and biocompatibility.
SN 2296-4185
YR 2020
FD 2020-06-16
LK https://hdl.handle.net/10668/28072
UL https://hdl.handle.net/10668/28072
LA en
DS RISalud
RD Apr 11, 2025