RT Journal Article T1 Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015-2018 A1 Gámbaro, Fabiana A1 Pérez, Ana Belén A1 Agüera, Eduardo A1 Prot, Matthieu A1 Martínez-Martínez, Luis A1 Cabrerizo, María A1 Simon-Loriere, Etienne A1 Fernandez-Garcia, Maria Dolores K1 Genomics K1 Enterovirus K1 Meningitis K1 Spain K1 RNA K1 Genómica K1 España K1 ARN AB New circulating Enterovirus (EV) strains often emerge through recombination. Upsurges of recombinant non-polio enteroviruses (NPEVs) associated with neurologic manifestations such as EVA71 or Echovirus 30 (E30) are a growing public health concern in Europe. Only a few complete genomes of EVs circulating in Spain are available in public databases, making it difficult to address the emergence of recombinant EVs, understand their evolutionary relatedness and the possible implication in human disease. We have used metagenomic (untargeted) NGS to generate full-length EV genomes from CSF samples of EV-positive aseptic meningitis cases in Southern Spain between 2015 and 2018. Our analyses reveal the co-circulation of multiple Enterovirus B (EV-B) types (E6, E11, E13 and E30), including a novel E13 recombinant form. We observed a genetic turnover where emergent lineages (C1 for E6 and I [tentatively proposed in this study] for E30) replaced previous lineages circulating in Spain, some concomitant with outbreaks in other parts of Europe. Metagenomic sequencing provides an effective approach for the analysis of EV genomes directly from PCR-positive CSF samples. The detection of a novel, disease-associated, recombinant form emphasizes the importance of genomic surveillance to monitor spread and evolution of EVs. PB Springer Nature YR 2021 FD 2021-11-02 LK http://hdl.handle.net/10668/3781 UL http://hdl.handle.net/10668/3781 LA en NO Gámbaro F, Pérez AB, Agüera E, Prot M, Martínez-Martínez L, Cabrerizo M, et al. Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015-2018. Sci Rep. 2021 Nov 2;11(1):21523 DS RISalud RD Apr 5, 2025