RT Journal Article
T1 Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study).
A1 Alastruey-Izquierdo, Ana
A1 Alcazar-Fuoli, Laura
A1 Rivero-Menéndez, Olga
A1 Ayats, Josefina
A1 Castro, Carmen
A1 García-Rodríguez, Julio
A1 Goterris-Bonet, Lidia
A1 Ibáñez-Martínez, Elisa
A1 Linares-Sicilia, María José
A1 Martin-Gomez, M Teresa
A1 Martín-Mazuelos, Estrella
A1 Pelaez, Teresa
A1 Peman, Javier
A1 Rezusta, Antonio
A1 Rojo, Susana
A1 Tejero, Rocio
A1 Anza, Diego Vicente
A1 Viñuelas, Jesús
A1 Zapico, Maria Soledad
A1 Cuenca-Estrella, Manuel
A1 the FILPOP2 Project from GEMICOMED (SEIMC) and REIPI, 
K1 Aspergillus
K1 Fusarium
K1 Mucorales
K1 Scedosporium
K1 TR34/L98H
K1 antifungal resistance
K1 aspergillosis
K1 azole resistance
K1 cryptic species
K1 cyp51A
AB Antifungal resistance is increasing by the emergence of intrinsically resistant species and by the development of secondary resistance in susceptible species. A previous study performed in Spain revealed levels of azole resistance in molds of between 10 and 12.7%, but secondary resistance in Aspergillus fumigatus was not detected. We used itraconazole (ITZ)-supplemented medium to select resistant strains. A total of 500 plates supplemented with 2 mg/liter of ITZ were sent to 10 Spanish tertiary hospitals, and molecular identification and antifungal susceptibility testing were performed. In addition, the cyp51A gene in those A. fumigatus strains showing azole resistance was sequenced. A total of 493 isolates were included in the study. Sixteen strains were isolated from patients with an infection classified as proven, 104 were isolated from patients with an infection classified as probable, and 373 were isolated from patients with an infection classified as colonization. Aspergillus was the most frequent genus isolated, at 80.3%, followed by Scedosporium-Lomentospora (7.9%), Penicillium-Talaromyces (4.5%), Fusarium (2.6%), and the order Mucorales (1%). Antifungal resistance was detected in Scedosporium-Lomentospora species, Fusarium, Talaromyces, and Mucorales Three strains of A. fumigatus sensu stricto were resistant to azoles; two of them harbored the TR34+L98H mechanism of resistance, and the other one had no mutations in cyp51A The level of azole resistance in A. fumigatus remains low, but cryptic species represent over 10% of the isolates and have a broader but overall higher range of antifungal resistance.
YR 2018
FD 2018-08-27
LK http://hdl.handle.net/10668/12639
UL http://hdl.handle.net/10668/12639
LA en
DS RISalud
RD Apr 19, 2025