RT Journal Article
T1 Flavonoid Phloretin Inhibits Adipogenesis and Increases OPG Expression in Adipocytes Derived from Human Bone-Marrow Mesenchymal Stromal-Cells
A1 Casado-Diaz, Antonio
A1 Rodriguez-Ramos, Angel
A1 Torrecillas-Baena, Barbara
A1 Dorado, Gabriel
A1 Quesada-Gomez, Jose Manuel
A1 Galvez-Moreno, Maria Angeles
K1 phloretin
K1 human mesenchymal stem cells
K1 adipogenesis
K1 obesity
K1 bone
K1 Activated receptor-gamma
K1 Diet-induced obesity
K1 In-vitro
K1 Glucose-transporter
K1 Stem-cells
K1 Ppar-alpha
K1 Differentiation
K1 Phlorhizin
K1 Apple
K1 Apoptosis
AB Phloretin (a flavonoid abundant in apple), has antioxidant, anti-inflammatory, and glucose-transporter inhibitory properties. Thus, it has interesting pharmacological and nutraceutical potential. Bone-marrow mesenchymal stem cells (MSC) have high differentiation capacity, being essential for maintaining homeostasis and regenerative capacity in the organism. Yet, they preferentially differentiate into adipocytes instead of osteoblasts with aging. This has a negative impact on bone turnover, remodeling, and formation. We have evaluated the effects of phloretin on human adipogenesis, analyzing MSC induced to differentiate into adipocytes. Expression of adipogenic genes, as well as genes encoding OPG and RANKL (involved in osteoclastogenesis), protein synthesis, lipid-droplets formation, and apoptosis, were studied. Results showed that 10 and 20 mu M phloretin inhibited adipogenesis. This effect was mediated by increasing beta-catenin, as well as increasing apoptosis in adipocytes, at late stages of differentiation. In addition, this chemical increased OPG gene expression and OPG/RANKL ratio in adipocytes. These results suggest that this flavonoid (including phloretin-rich foods) has interesting potential for clinical and regenerative-medicine applications. Thus, such chemicals could be used to counteract obesity and prevent bone-marrow adiposity. That is particularly useful to protect bone mass and treat diseases like osteoporosis, which is an epidemic worldwide.
PB Mdpi
YR 2021
FD 2021-11-01
LK https://hdl.handle.net/10668/25643
UL https://hdl.handle.net/10668/25643
LA en
DS RISalud
RD Apr 17, 2025