RT Journal Article
T1 Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose Tissue or Umbilical Cord.
A1 Clemente, Luis Felipe
A1 Hernáez, María Luisa
A1 Ramos-Fernández, Antonio
A1 Ligero, Gertrudis
A1 Gil, Concha
A1 Corrales, Fernando José
A1 Marcilla, Miguel
AB Currently, 14% of the human proteome is made up of proteins whose existence is not confirmed by mass spectrometry. We performed a proteomic profiling of human mesenchymal stem cells derived from adipose tissue or umbilical cord (PRIDE accession number: PXD009893) and identified peptides derived from 13 of such missing proteins. Remarkably, we found compelling evidence of the expression of hyaluronan synthase 1 (NX_Q92839-1) and confirmed its identification by the fragmentation of four heavy-labeled peptides that coeluted with their endogenous light counterparts. Our data also suggest that mesenchymal stem cells constitute a promising source for the detection of missing proteins.
YR 2018
FD 2018-07-06
LK http://hdl.handle.net/10668/12675
UL http://hdl.handle.net/10668/12675
LA en
DS RISalud
RD Jul 31, 2025