RT Journal Article
T1 Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement.
A1 López-Ruiz, Elena
A1 Venkateswaran, Seshasailam
A1 Perán, Macarena
A1 Jiménez, Gema
A1 Pernagallo, Salvatore
A1 Díaz-Mochón, Juan J
A1 Tura-Ceide, Olga
A1 Arrebola, Francisco
A1 Melchor, Juan
A1 Soto, Juan
A1 Rus, Guillermo
A1 Real, Pedro J
A1 Diaz-Ricart, María
A1 Conde-González, Antonio
A1 Bradley, Mark
A1 Marchal, Juan A
AB Decellularized vascular scaffolds are promising materials for vessel replacements. However, despite the natural origin of decellularized vessels, issues such as biomechanical incompatibility, immunogenicity risks and the hazards of thrombus formation, still need to be addressed. In this study, we coated decellularized vessels obtained from porcine carotid arteries with poly (ethylmethacrylate-co-diethylaminoethylacrylate) (8g7) with the purpose of improving endothelial coverage and minimizing platelet attachment while enhancing the mechanical properties of the decellularized vascular scaffolds. The polymer facilitated binding of endothelial cells (ECs) with high affinity and also induced endothelial cell capillary tube formation. In addition, platelets showed reduced adhesion on the polymer under flow conditions. Moreover, the coating of the decellularized arteries improved biomechanical properties by increasing its tensile strength and load. In addition, after 5 days in culture, ECs seeded on the luminal surface of 8g7-coated decellularized arteries showed good regeneration of the endothelium. Overall, this study shows that polymer coating of decellularized vessels provides a new strategy to improve re-endothelialization of vascular grafts, maintaining or enhancing mechanical properties while reducing the risk of thrombogenesis. These results could have potential applications in improving tissue-engineered vascular grafts for cardiovascular therapies with small caliber vessels.
YR 2017
FD 2017-03-24
LK http://hdl.handle.net/10668/11004
UL http://hdl.handle.net/10668/11004
LA en
DS RISalud
RD Jun 1, 2025