RT Journal Article
T1 Generation and Evaluation of Novel Biomaterials Based on Decellularized Sturgeon Cartilage for Use in Tissue Engineering.
A1 Ortiz-Arrabal, Olimpia
A1 Carmona, Ramón
A1 García-García, Óscar-Darío
A1 Chato-Astrain, Jesús
A1 Sánchez-Porras, David
A1 Domezain, Alberto
A1 Oruezabal, Roke-Iñaki
A1 Carriel, Víctor
A1 Campos, Antonio
A1 Alaminos, Miguel
K1 cartilage
K1 decellularization
K1 sturgeon
K1 tissue engineering
AB Because cartilage has limited regenerative capability, a fully efficient advanced therapy medicinal product is needed to treat severe cartilage damage. We evaluated a novel biomaterial obtained by decellularizing sturgeon chondral endoskeleton tissue for use in cartilage tissue engineering. In silico analysis suggested high homology between human and sturgeon collagen proteins, and ultra-performance liquid chromatography confirmed that both types of cartilage consisted mainly of the same amino acids. Decellularized sturgeon cartilage was recellularized with human chondrocytes and four types of human mesenchymal stem cells (MSC) and their suitability for generating a cartilage substitute was assessed ex vivo and in vivo. The results supported the biocompatibility of the novel scaffold, as well as its ability to sustain cell adhesion, proliferation and differentiation. In vivo assays showed that the MSC cells in grafted cartilage disks were biosynthetically active and able to remodel the extracellular matrix of cartilage substitutes, with the production of type II collagen and other relevant components, especially when adipose tissue MSC were used. In addition, these cartilage substitutes triggered a pro-regenerative reaction mediated by CD206-positive M2 macrophages. These preliminary results warrant further research to characterize in greater detail the potential clinical translation of these novel cartilage substitutes.
SN 2227-9059
YR 2021
FD 2021-07-04
LK https://hdl.handle.net/10668/28139
UL https://hdl.handle.net/10668/28139
LA en
DS RISalud
RD Apr 5, 2025