RT Journal Article
T1 Methodology for Y Chromosome Capture: A complete genome sequence of  Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads.
A1 Alvarez-Cubero, M J
A1 Santiago, O
A1 Martínez-Labarga, C
A1 Martínez-García, B
A1 Marrero-Díaz, R
A1 Rubio-Roldan, A
A1 Pérez-Gutiérrez, A M
A1 Carmona-Saez, P
A1 Lorente, J A
A1 Martinez-Gonzalez, L J
AB This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin-biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences.
YR 2018
FD 2018-06-21
LK http://hdl.handle.net/10668/12631
UL http://hdl.handle.net/10668/12631
LA en
DS RISalud
RD Apr 6, 2025