RT Journal Article
T1 Improved integrative analysis of the thiol redox proteome using filter-aided sample preparation.
A1 Bonzon-Kulichenko, Elena
A1 Camafeita, Emilio
A1 Lopez, Juan Antonio
A1 Gomez-Serrano, Maria
A1 Jorge, Inmaculada
A1 Calvo, Enrique
A1 Nuñez, Estefania
A1 Trevisan-Herraz, Marco
A1 Bagwan, Navratan
A1 Barcena, Jose Antonio
A1 Peral, Belen
A1 Vazquez, Jesus
K1 Adipose tissue
K1 Cys oxidation
K1 FASILOX
K1 Filter-aided sample preparation
K1 Sexual dimorphism
K1 Thiol redox proteome
AB Changes in the oxidation state of protein Cys residues are involved in cell signalling and play a key role in a variety of pathophysiological states. We had previously developed GELSILOX, an in-gel method that enables the large-scale, parallel analysis of dynamic alterations to the redox state of Cys sites and protein abundance changes. Here we present FASILOX, a further development of the GELSILOX approach featuring: i) significantly increased peptide recovery, ii) enhanced sensitivity for the detection of Cys oxidative alterations, and iii) streamlined workflow that results in shortened assay duration. In mitochondria isolated from the adipose tissue of obese, diabetic patients, FASILOX revealed a sexually dimorphic trait of Cys oxidation involving mainly mitochondrial oxidative phosphorylation complexes. These results provide the first evidence for a decreased efficiency in the antioxidant response of men as compared to women.
PB Elsevier
YR 2019
FD 2019-12-20
LK http://hdl.handle.net/10668/14875
UL http://hdl.handle.net/10668/14875
LA en
NO Bonzon-Kulichenko E, Camafeita E, López JA, Gómez-Serrano M, Jorge I, Calvo E, et al. Improved integrative analysis of the thiol redox proteome using filter-aided sample preparation. J Proteomics. 2020 Mar 1;214:103624
NO This study was supported by competitive grants from the SpanishMinistry of Science, Innovation and Universities (BIO2015-67580-P,PGC2018-097019-B-I00 and RTI2018-093864-B-100), the Ministry ofEconomy, Industry and Competitiveness (BFU2016-80006-P) throughthe Carlos III Institute of Health-Fondo de Investigación Sanitaria grantPRB3 (IPT17/0019 - ISCIII-SGEFI / ERDF, ProteoRed), the FundacióMaratóTV3 (grant 122/C/2015) and “la Caixa” Banking Foundation(project code HR17-00247). The CNIC is supported by the Instituto deSalud Carlos III (ISCIII), the Ministerio de Ciencia, Innovación yUniversidades (MCNU) and the Pro CNIC Foundation, and is a SeveroOchoa Center of Excellence (SEV-2015-0505).
DS RISalud
RD Apr 17, 2025