RT Journal Article T1 "An End to a Means": How DNA-End Structure Shapes the Double-Strand Break Repair Process A1 Serrano-Benítez, Almudena A1 Cortés-Ledesma, Felipe A1 Ruiz, Jose F. K1 DNA double strand break (DSB) K1 Non-homologous DNA end joining K1 ATM K1 DNA-PK catalytic subunit K1 Genome instability K1 Roturas del ADN de doble cadena K1 Reparación del ADN por unión de extremidades K1 Proteínas de la ataxia telangiectasia mutada K1 Proteína quinasa activada por ADN K1 ADN AB Endogenously-arising DNA double-strand breaks (DSBs) rarely harbor canonical 5'-phosphate, 3'-hydroxyl moieties at the ends, which are, regardless of the pathway used, ultimately required for their repair. Cells are therefore endowed with a wide variety of enzymes that can deal with these chemical and structural variations and guarantee the formation of ligatable termini. An important distinction is whether the ends are directly "unblocked" by specific enzymatic activities without affecting the integrity of the DNA molecule and its sequence, or whether they are "processed" by unspecific nucleases that remove nucleotides from the termini. DNA end structure and configuration, therefore, shape the repair process, its requirements, and, importantly, its final outcome. Thus, the molecular mechanisms that coordinate and integrate the cellular response to blocked DSBs, although still largely unexplored, can be particularly relevant for maintaining genome integrity and avoiding malignant transformation and cancer. PB Frontiers YR 2020 FD 2020-01-10 LK http://hdl.handle.net/10668/3746 UL http://hdl.handle.net/10668/3746 LA en NO Serrano-Benítez A, Cortés-Ledesma F, Ruiz JF. "An End to a Means": How DNA-End Structure Shapes the Double-Strand Break Repair Process. Front Mol Biosci. 2020 Jan 10;6:153 DS RISalud RD Feb 15, 2025