RT Journal Article
T1 Modulation of MDR1 and MRP3 Gene Expression in Lung Cancer Cells after Paclitaxel and Carboplatin Exposure
A1 Melguizo, Consolación
A1 Prados, José
A1 Luque, Raquel
A1 Ortiz, Raúl
A1 Caba, Octavio
A1 Álvarez, Pablo J
A1 Gonzalez, Bestriz
A1 Aranega, Antonia
K1 resistance
K1 lung cancer
K1 MDR
K1 MRP
K1 paclitaxel
K1 carboplatin
K1 Genes MDR
K1 Neoplasias Pulmonares
K1 Resistencia a Antineoplásicos
AB Carboplatin-paclitaxel is a reference regimen in the treatment of locally advanced or disseminated non-small cell lung cancer (NSCLC). This paper discusses the multidrug resistance developed with this drug combination, which is one of the major obstacles to successful treatment. In order to understand and overcome the drug resistance pattern of NSCLC after carboplatin plus paclitaxel exposure, levels of mRNA expression of multidrug resistance 1 (MDR1) and multidrug resistance-associated protein 3 (MRP3) were investigated in primary NSCLC cell lines (A-549 and A-427) and a metastasis-derived NSCLC cell line (NODO). Our results showed that exposure of the three NSCLC lines to plasma concentrations of paclitaxel (5 μM) produced an increase in MDR1 expression, while MRP3 showed no alteration in expression. By contrast, the same cells exposed to carboplatin plasma concentrations (30 μM) showed overexpression of MRP3. In these cells, MDR1 showed no expression changes. Interestingly, the combination of both paclitaxel and carboplatin caused increased expression of the MDR1 drug resistance gene rather than the individual treatments. These results suggest that carboplatin and paclitaxel may induce drug resistance mediated by MDR1 and MRP3, which may be enhanced by the simultaneous use of both drugs.
PB MDPI
YR 2012
FD 2012-12-05
LK http://hdl.handle.net/10668/774
UL http://hdl.handle.net/10668/774
LA en
NO Melguizo C, Prados J, Luque R, Ortiz R, Caba O, Álvarez PJ et al. Modulation of MDR1 and MRP3 Gene Expression in Lung Cancer Cells after Paclitaxel and Carboplatin Exposure. International Journal of Molecular Sciences. 2012; 13(12):16624-16635.
DS RISalud
RD Apr 11, 2025